摘要
利用鼠鼠杂交瘤细胞（ＷＵＴ３）研究培养基中不同浓度的肉毒碱对细胞生长、葡萄糖利用速率、乳酸、氨、单克隆抗体生成速率的影响。结果表明，增加肉毒碱浓度（从０％～０．０４％），细胞生长速率增加；葡萄糖消耗，乳酸生成，氨生成速率降低；单抗生成速率增加，当肉毒碱浓度达００４％时，细胞最高密度增加１／３，葡萄糖消耗速率减少１／３，氨生成速率降低约４０％，单抗生成速率增加约５０％，乳酸生成速率降低５０％，消耗的葡萄糖进入ＴＣＡ循环比例增加，当葡萄糖浓度降低到０４ｇ／Ｌ时。
The effects caused by carnitine of different concentration on cell growth,glucose utilization rate,lactic acid,ammonia production and McAb production rate have been studied by using mouse mouse hybridoma WUT3 cells.It is shown that with the increase of carnitine concentration (ranged from 0%～0.04%)the rate of cell growth is increased,however the glucose utilization,lactic acid and ammonia production rate are decreased.When the carnitine concentration is risen to 0.04%,the maximum cell density is increased one third ,the rate of glucose utilization is decreased one third,lactic acid production rate is decreased 50%,and ammonia production rate is decreased about 40%.But monoclonal antibody rate is increased about 50%,and the proportion of used glucose entering TCA circle is increased.When the glucose concentration is decreased to 0.4g/L,it will not be utilized further.
出处
《生物工程学报》
CAS
CSCD
北大核心
1996年第S1期72-75,共4页
Chinese Journal of Biotechnology
基金
国家85攻关资助课题
关键词
肉毒碱
单克隆抗体
葡萄糖
乳酸
氨
Carnitine,monoclonal antibody,glucose,lactic acid,ammonia
