摘要
本实验采用研磨法制备蔗糖酶，酶活性达1．575×105u·ml-1，并对其酶学性质进行了研究．测得其表观Km值约为0．015mol·1-1；初速度反应时间为0-10min；最适酶量为3．152×103-7．88×103u·mmol-1蔗糖；最适底物浓度为0．5mol·1-1；最适pH值在NaAcHAc体系中为4．4；最适反应温度为50℃．
In the title the sucrase was prepared by Griding methed. The enzyme activity was 1.575×105u·mol-1. Its sucrase properties were also studied. The sucrase followed typical Michaelis-Meten kinetics with apparent Km of 0.015mol·1-1 for sucrose, the time of preliminary velocity was 0-10min, the optimum sucrose quantity was 3. 152×103-7.88 ×103u·mmol-1 enzyme, the optimu optimu substrate cubstrate was 0. 5mol·1-1, the optimun pH was 4 .4 in NaAc-HAc, the optimun temperatUre was 50℃.
出处
《湛江师范学院学报》
1997年第2期57-60,共4页
Journal of Zhanjiang Normal College
关键词
酵母
蔗糖酶
分离
酶学性质
yeast sucrase separation enzyme properties
