摘要
It is not certain whether the myocyte contractile function in the heartfailure is normal according to recent some studies,therefore the contractile functionof the myocytes with the heart failure should be studied. The myocyte heart failuremodel was set up by the method of stretching the cultured myocytes,and the myocytes with and without the heart failure were measured the contractile mechanicscharacteristic,the parameters being the maximal contractive velocity of the myocyte(Vmax), the maximal shortening length of myocyte contraction (ΔLmax) and themaximal decrease of the myocyte size during contraction (ΔSmax), by the videoedgedetector system. And then the protein genes (including α-MHC and β-MHC)expression of these myocytes were quantitatively measured by the dot blotting. Thecorrelative and regressive analysis were used to analyze the relationship between themyocyte contractile mechanics characteristic and the contractile protein genes expression level. The results showed that the contractile mechanics parameters,Vmax=41．5±6．0(μm/sec), △Lmax= 10．6±1．9 (% ) and △Smax= 14．3± 2．3 (% ), in themyocytes with the heart failure are statistically significant lower than the normalmyocytes (P<0．01), whose parameters are Vmax=67．9±6．7 (μm/sec), △Lmax=17．7±1．6 (% ) and △Smax=21．5±2．4 (%). The myocyte contractile protein gene (αMHC) expression level in the heart failure myocytes is statistically significant lowerthan that in the normal myocytes,but β-MHC gene expression level is statisticallysignificant higher than the normal myocytes. The α-MllC gene expression level is apositive correlation coefficient with the myocyte contractile mechanics parametersseparately,covering Vmax,△Lmax and △max; but the β- MHC gene expression levelis a negative correlation coefficietn with the myocyte contractile mechanics parameters separately.
It is not certain whether the myocyte contractile function in the heartfailure is normal according to recent some studies,therefore the contractile functionof the myocytes with the heart failure should be studied. The myocyte heart failuremodel was set up by the method of stretching the cultured myocytes,and the myocytes with and without the heart failure were measured the contractile mechanicscharacteristic,the parameters being the maximal contractive velocity of the myocyte(Vmax), the maximal shortening length of myocyte contraction (ΔLmax) and themaximal decrease of the myocyte size during contraction (ΔSmax), by the videoedgedetector system. And then the protein genes (including α-MHC and β-MHC)expression of these myocytes were quantitatively measured by the dot blotting. Thecorrelative and regressive analysis were used to analyze the relationship between themyocyte contractile mechanics characteristic and the contractile protein genes expression level. The results showed that the contractile mechanics parameters,Vmax=41．5±6．0(μm/sec), △Lmax= 10．6±1．9 (% ) and △Smax= 14．3± 2．3 (% ), in themyocytes with the heart failure are statistically significant lower than the normalmyocytes (P<0．01), whose parameters are Vmax=67．9±6．7 (μm/sec), △Lmax=17．7±1．6 (% ) and △Smax=21．5±2．4 (%). The myocyte contractile protein gene (αMHC) expression level in the heart failure myocytes is statistically significant lowerthan that in the normal myocytes,but β-MHC gene expression level is statisticallysignificant higher than the normal myocytes. The α-MllC gene expression level is apositive correlation coefficient with the myocyte contractile mechanics parametersseparately,covering Vmax,△Lmax and △max; but the β- MHC gene expression levelis a negative correlation coefficietn with the myocyte contractile mechanics parameters separately.
