摘要
目的探讨胃癌组织中膜型基质金属蛋白酶一1（MT1一MMP）和RECK蛋白表达状况和两者之间的关系，及其表达与胃癌临床诊断之间的关系。方法采用免疫组织化学法（两步法）对胃癌切除标本进行研究。结果在44例胃癌标本中，有37（84．1％）例MT1-MMP表达呈阳性反应，31（70．5％）例RECK免疫组化为阳性。MT1-MMP表达与胃癌分化具有一定的关系，低分化组织标本中，MT1一MMP表达较多，而中、高分化组织标本中，MT1-MMP表达相对较弱，具有统计学意义（P=0．015）。胃癌中MT1-MMP表达与肿瘤细胞浸润深度相关（P=0．007）。但MT1-MMP表达与性别以及有无淋巴结转移之间未见统计学差异。与此相反，在胃癌标本中，高分化标本中RECK蛋白表达相对较多，中分化标本其次，在低分化标本中表达较少。RECK表达与肿瘤分化具有统计学意义（P：0．006）。RECK表达与性别、肿瘤细胞浸润深度以及有无淋巴结转移无关，且与MT1-MMP表达之间亦无统计学意义。结论MT1-MMP过表达在胃癌分化、转移中发挥着重要的作用，而RECK的表达有益于胃癌向高分化发展。
Objective To examine the expression of membrane-type-1 matrix metalloproteinase （MT1-MMP） and reversion-inducing cysteine-rich protein with Kazal motifs （RECK） in gastric carcinoma, and investigate its clinical significance, at the same time analyze the correlation between MT1-MMP and RECK expression. Methods MT1-MMP and RECK expression in surgically resected tissue samples of gastric carcinoma was examined by immunohistochemical method （two-step method） , and its correlation with clinicopathological factors was analyzed. Results Among the 44 gastric carcinoma samples, 37 （84. 1% ） were stained positive for MT1-MMP, and 31 （70.5%） for RECK. The expression of MT1-MMP was much higher in poorly differentiated gastric carcinoma samples than moderately and well-differentiated samples （P = 0. 015）. The expression level of MT1-MMP was associated with invasive depth of tumor cells （P = 0. 007） , but no difference between sex and lymph node metastasis. On the contrary, the well-differentiated samples showed higher expression of RECK than poorly and moderately differentiated gastric carcinoma samples （P = 0. 006）. The expression level of RECK did not correlate with sex, lymph node metastasis and invasive depth of tumor cells. RECK expression showed no relation to MT1-MMP expression in the gastric carcinoma. Conclusion Overexpression of MT1-MMP in gastric carcinoma may play an important role during tumor differentiation and metastasis, the RECK protein may have positive effects on the tumor differentiation.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2011年第5期364-367,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
基质金属蛋白酶
半胱氨酸蛋白酶抑制剂
胃肠肿瘤
免疫组织化学
Matrix metalloproteinases
Cysteine proteinase inhibitors
Gastrointestinal neoplasms
Immunohistochemistry
