摘要
采用反相高效液相色谱法测定吡嗪酰胺片的含量。选用PRODIGY ODS(250×4.60mm.5μm)色谱柱,以邻甲苯磺酰胺为内标,甲醇-水-冰乙酸(45:55:1,以三乙胺调pH=3.5)为流动相,检测波长为268nm,测得线性范围4～12μg,r=0.9999,平均回收率为99.8％,RSD为0.8％(n=3)。
A RP - HPLC method was developed for the determination of Pyrazinamide tablets. The analytical column was packed with PRODIGY ODS (250×4. 60mm, 5μm) ,O - tolylsulfonilamidum was selected as the internal standard. The mobile phase consisted of methanol -water -glacial acetic acid(45 : 55 : 1) (adjust to pH3. 5 with triethylamine). The detection wavelength was 268nm,The calibration curve was linear within the range of 4 - 12μg and the correlation coefficient was 0. 9999. the average recovery was 99. 8% ,and the RSD was 0. 8% (n = 3).
出处
《药学与临床研究》
1999年第3期17-18,共2页
Pharmaceutical and Clinical Research
