摘要
应用ERIC2,Ap1和Ap7三种随机引物进行26株临床分离金黄色葡萄球菌的随机扩增多态性DNA分型研究。结果显示:三种引物均能有效地进行金黄色葡萄球菌的分子生物学分型,分别分为9型、6型和10型,而引物Ap7具有更好的可分型性。
The randomly amplified polymorphic DNA(RAPD) was used to type the 26 clinically isolated strains of staphylococcus aureus. The results were that all three primers of this method, ERIC 2, Ap1, and Ap7 on molecular typing of staphylococcus aureus and identified 9,6, and 10 genotypes, respectively; the primer Ap7 produced better results than the other primers. The present study suggests that RAPD is a simple and rapid method and has a practical value in the molecular typing of staphylococcus aureus.
出处
《中南大学学报(医学版)》
CAS
CSCD
1999年第1期20-22,共3页
Journal of Central South University :Medical Science