摘要
Objective To induce antibody response against E2 glycoprotein derived from a Chinese genotype Ⅲ/2a isolate of hepatitis C virus (HCV) in BALB/C mice by plasmid DNA based immunization. Methods Plasmid p3W14 containing E2/NS1 gene derived from a Chinese genotype III/2a isolate of HCV, which was verified to express 70 kDa E2 glycoprotein in NIH3T3 cells, was used for direct intramuscular injection in BALB/C mice. Specific antibody to E2 glycoprotein was detected by recombinant E2 protein. Results Specific anti E2 antibody could be detected in mice inoculated with p3W14(5/6). Titer of antibody ranged from 1∶15 to 1∶120. Conclusion Successfully inducing anti E2 antibody in mice by plasmid DNA based immunization containing E2/NS1 gene from a Chinese genotype Ⅲ/2a isolate of HCV is the first time in China.
Objective To induce antibody response against E2 glycoprotein derived from a Chinese genotype Ⅲ/2a isolate of hepatitis C virus (HCV) in BALB/C mice by plasmid DNA based immunization. Methods Plasmid p3W14 containing E2/NS1 gene derived from a Chinese genotype III/2a isolate of HCV, which was verified to express 70 kDa E2 glycoprotein in NIH3T3 cells, was used for direct intramuscular injection in BALB/C mice. Specific antibody to E2 glycoprotein was detected by recombinant E2 protein. Results Specific anti E2 antibody could be detected in mice inoculated with p3W14(5/6). Titer of antibody ranged from 1∶15 to 1∶120. Conclusion Successfully inducing anti E2 antibody in mice by plasmid DNA based immunization containing E2/NS1 gene from a Chinese genotype Ⅲ/2a isolate of HCV is the first time in China.
