摘要
对肾上腺素自氧化反应进行了研究， 发现反应中间产物能发出较强的荧光， 其生成量受超氧化物歧化酶的抑制． 据此建立了分析超氧化物歧化酶的新方法． 该方法线性范围为０ ～５２－６ｎｇ／ｍ Ｌ， 检测限为８－５６ｎｇ／ｍＬ， 相对标准偏差为１－６ ％ （ ｎ ＝ ６） ．
A fluorescent method is proposed for the determination of superoxide dismutase (SOD) based on epinephrine autoxidation.We found that the intermediate product of the autoxidation process of epinephrine autoxidation.Can fluoresce rather strongly at 533 nm when excited at 333 nm. The amount of SOD is linearly dependent on the extent of the decrease of the fluorescence of the immediate.Under optimum conditions, the calibration graph was linear over a range of 0～53 ng/mL SOD.The detection limit was 8 6 ng/mL SOD.The relative deviation was 1 6% for 26 ng/mL SOD ( \%n\%=6 ).
出处
《福州大学学报（自然科学版）》
CAS
CSCD
1999年第S1期65-66,共2页
Journal of Fuzhou University(Natural Science Edition)
基金
国家自然科学基金!(29677014)
