摘要
目的探讨虫草菌丝、丹参抗肝纤维化的作用机理。方法虫草菌丝与丹参流浸膏分别给大鼠经口灌胃给药后分离药物血清，观察药物血清对体外传代活化的大鼠肝星状细胞α－平滑肌肌动蛋白（SMactin，SMA）表达、[3H]TdR及[3H]脯氨酸掺入，TGFβ1、I型前胶原mRNA表达及其蛋白生成量的影响。结果丹参抑制HSC的SMA表达、I型胶原生成量及细胞内[3H]脯氨酸掺入的作用显著，分别为对照组的39.8％，42．7％与38．9％（P＜0．01；前2项显著低于虫草菌丝组，P＜0．05），尚可抑制细胞1型前胶原＜mRNA，TGFβ1＜mRNA及其蛋白的表达（P＜0．01）；虫草菌丝对上述指标也均有抑制作用，以抑制TGFβ1＜mRNA及其蛋白表达的作用尤著，分别为对照组的47．7％和21．1%(P＜0．01）；显著低于丹参组，P＜0．05。结论丹参具有较强的抑制HSC活化与胶原合成的作用，抑制胶原合成主要作用于前胶原转录后的水平；虫草菌丝的主要作用点抑制HSC的TGFβ1mRNA表达与自分泌。
Objective To investigate the mechanisms of cordyceps mycelium and salvia mitiorrhizae actions for anti-liver fibrosis. Methods The normal rats were fed on cordyceps mycelium and salvia mitiorrnizae respectively, then the rat sera were collected and incubated with subcultured hepatic stellatecells (HSC). HSC α-SM actin (SMA) expression, [3H]TdR and [3H]Proline incorporation, mRNA and protein production of type I collagen, mRNA and protein production of Transforming Growth Factor β1(TGFβ1) were observed. The incoopration of [3H]TdR and [3H]Pro, procollagen type I mRNA expression, collagen production, and hepatocyte albumin production were observed. Results Salvia mitiorrhizae decreased HSC SMA expression, type I collagen production and intracellular [3H]TdR incorporation remarkably, the ratio of control were 39.8%, 42.7% and 38.9% (p<0.01 vs. control. The former 2 indexes were obviously lower than the ones of cordyceps mycelium, P<0.05), also inhibited mRNA and protein expression of type I collagen and TGFβ1 (P<0.05). Cordyceps mycelium had similar effects on the above indexes, but especially decreased HSC TGFβ1 mRNA and protein expression strongly, the ration of control were 47.7% and 21.1% respectively (p<0.01 vs. Control, P<0.05 vs. Salvia mitiorrhizae treated group).Conclusions Salvia mitiorrhizae had the stronger action on inhibition of HSC activation cellagen production, mainly on precollopn bine posttranscription levels. Cordyceps mycelium mainly inhibited HSC TGFβ1 mRNA expression and autocrine. [
出处
《中华肝脏病杂志》
CAS
CSCD
1999年第S1期24-26,共3页
Chinese Journal of Hepatology
基金
国家杰出青年科学基金!39825128
关键词
药
中草
转化生长因子Β1
肝星状细胞
Ⅰ型胶原
: Drugs, Chinese herbal Transforming Growth Factor β1 Hepatic stellate cellType I collage
