摘要
采用聚丙烯酰胺凝胶电泳技术分析了濒危植物尖叶拟船叶藓7个自然居群的过氧化物同工酶．结果表明：尖叶拟船叶藓在13个位点表现酶带．其中 Pod-4、Pod-6、Pod-7为7个居群共有,是尖叶拟船叶藓的特征带；1号居群酶带最多,为12条,除与其他居群共有的3条主带外,还出现了其特有的3务酶带；尖叶拟船叶藓存在哑等位基因现象,有两个稀有基因 Pod-5和 Pod-11．3号和5号、4号和6号有较强的同源性．据此认为尖叶拟船叶藓具有较丰富的遗传多样性．
POD isoenzymes in seven natural populations of Dolichomitriopsis diversiformis were analyzed by using poly-acrylamide gel electrophoresis(PAGE).Results showed that there were 13 types of isoenzymes in Dolichomitriopsis diversiformis.In them,Pod-4,Pod-6 and Pod-7 existed in any populations,and they should be the characteristics of Dolichomitriopsis diversiformis.Population 1 had 12 isoenzymes sites,besides 3 char- acteristic sites,there were 3 sepcial sites other populations not had.Otherwise,sub-allele phenomenon occurred in Dolichomitriopsis diversiformis for Pod-5 and Pod-11 were less-observed.Results also revealed that popula- tion 3 and 5,population 4 and 6,was homologous.Based on these results it was concluded that Dolichomitri- opsis diversiformis exhibits genetic diversity.
出处
《生命科学研究》
CAS
CSCD
2005年第S2期78-81,共4页
Life Science Research
基金
国家自然科学基金(30470181)
关键词
尖叶拟船叶藓
过氧化物同工酶
酶谱分析
Dolichomitriopsis diversiformis(Mitt.)Nog.
peroxidase(POD)isoenzymes
map analysis
