摘要
目的探讨蛋白激酶C（PKC）激动剂佛波醇酯（PMA）对MES 23.5细胞中铁调节蛋白1（IRP1）的激活作用。方法采用MTT法观察细胞的存活率,Western Blot法检测IRP1和铁转出蛋白（Fpn1）的表达变化。结果 0.01~2.00μmol/L的PMA作用MES 23.5细胞24 h后,细胞存活率无明显变化（F=3.050,P〉0.05）。0.20μmol/L的PMA作用MES 23.5细胞24 h后,IRP1表达明显上调（t=4.501,P〈0.01）,Fpn1表达明显下调（t=5.174,P〈0.01）。结论 PMA在多巴胺能神经细胞系MES 23.5细胞中能明显激活IRP1。
Objective To study the activation of phorbol ester,agonist of protein kinase C（PKC） on iron regulatory protein-1（IRP1） in dopaminergic cell line MES 23.5 cells. Methods Cell viability was assessed by MTT.IRP1 and Fpn1 levels were investigated by Western Blot. Results After treatment with 0.01-2.00 μmol/L PMA for 24 hours,the survival of MES23.5 cells was not changed（F=3.050,P0.05）.After treatment with 0.20 μmol/L PMA for 24 hours,the expression of IRP1 upregulated（t=4.501,P0.01）,and Fpn1 expression down-regulated（t=5.174,P0.01）. Conclusion IRP1 can be activated by PMA in dopaminergic MES 23.5 cells.
出处
《青岛大学医学院学报》
CAS
2011年第5期379-381,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
国家自然科学基金资助项目(30930036
30600-190)
山东省教育厅资助项目(G09LC09)
青岛市科技局项目(09-1-1-29-nsh)
