摘要
采用毛细管电泳/电容耦合非接触式电导(CE/C4D),以18 mmol/L柠檬酸+6 mmol/L氨水+12 mg/L羟丙基甲基纤维素(HPMC)+35 mmol/L羟丙基-β-环糊精(HP-β-CD)为电泳运行液,熔融石英毛细管(50μm i.d.×45 cm,leff=40 cm),正高压(+15 kV)分离,手性药物氨氯地平对映体在该分离条件下可获得良好的基线分离,其线性范围为2.0～40 mg/L,检出限为0.5 mg/L。考察了电泳运行液组成、二元手性选择剂(HP-β-CD和HPMC)浓度、进样方式和样品基质等对灵敏度和分离度的影响。将方法用于市售外消旋和左旋氨氯地平片剂中氨氯地平对映体的分离测定,结果满意。
A novel enantioseparation method for amlodipine by high performance capillary electrophoresis with capaeitively coupled contactless conductivity detection （C4D） using binary chiral selectors was developed. The enantiomers of amlodipine were baseline separated in 20 min with an uncoated fused-silica capillary（50 μm i. d. ×45 cm, letr =40 cm） under the following optimum conditions： 18 mmol/L citric acid ＋ 6 mmol/L NH3 · H2O ＋ 12 mg/L hydroxypropyl methyl cellulose（HPMC） ＋ 35 mmol/L hydroxypropyl-β-cyclodextrin （ HP-β-CD ） as background electrolyte, separation voltage ： ＋ 15 kV, injection voltage： 13 kV, injection time： 10 s. The calibration curve of the enantiomers showed a good linearity in the range of 2.0 - 40 mg/L with correlation coefficient more than 0. 995. The detection limit of the enantiomers was 0.5 mg/L. Effects of several factors on resolutions, such as composition of running buffer, pH value, separation voltage and injection method, were investigated. The efficacy of the HP-β-CD and HPMC were discussed. The results indicated that the proposed method was simple, low cost, rapid and reproducible, and was successfully applied to monitor the enantiomers of amlodipine in the commercial amlodipine tablets and levoamlodipine tablets.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第11期1316-1319,共4页
Journal of Instrumental Analysis
基金
广东省自然科学基金资助项目(031589)
2010年中山大学"创新实验"基金资助项目
关键词
非接触式电导
毛细管电泳
手性药物分析
二元手性选择剂
氨氯地平
capacitively coupled contactless conductivity detection
capillary electrophoresis
chiral drugs analysis
binary chiral selectors
. amlodipine
