摘要
作为一种极为灵敏、快速的新型生物检测技术,光学细胞传感器在生物医学领域的研究应用备受关注,成为当今生物分析化学领域的研究前沿和热点。它是以细胞作为传感元件来研究信号识别、传导和指示的过程,在毒性物质、病原体等外界条件作用下,研究细胞中活性分子及其生理条件的变化,通过光学信号的变化定量分析细胞膜表面分子、胞内酶分子及微环境中活性氧、谷胱甘肽、pH值等的变化,从而获取细胞功能性信息,这对疾病的诊断和治疗具有重要的指导意义。该文主要介绍以纳米粒子作为光学探针的光学细胞传感器在癌细胞研究中的最新进展。
Optical biosensors based on different optical phenomena have been used widely over the past decade to analyse biomolecular interactions. With the advent of the nanoparticle technology, the probing of individual living ceils, .and the measuring of chemical species in specific locations within the cell for living cell sensing have become possible. Optical biosensors based on nanoparticles offer high sensitivity, specificity and rapid detection in the study of cancer cell or biomarker. In this paper, the applications of nanoparticles-based optical biosensor for the specific identification of molecules on the cell membrane surface for the study Of cancer cells, the research of enzyme molecule in cancer cells and intracellular and the reactive oxygen, glutathione and physiological pH value in the smallest environment are reviewed. The prospect of this novel technique is also discussed.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第11期1207-1215,共9页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(21071064)
科技部973计划资助项目(2010CB833603)
关键词
细胞传感器
光学检测
纳米粒子
光学探针
癌细胞
cell-based biosensors
optical detection
nanoparticles
optical probe
cancer cells
