摘要
研究高温对黄瓜生长的影响,筛选简单可靠的耐热性鉴定指标,对于提高耐热育种效率具有重要意义。将5个黄瓜自交系分别在38℃和42℃热胁迫下处理3d,测定了处理前后黄瓜幼苗的生理指标及叶绿素荧光参数的变化,结果表明:热害指数较高的自交系,其细胞膜透性也较大;随着热胁迫温度的升高,超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性逐渐上升,而过氧化物酶(POD)和过氧化氢酶(CAT)活性则持续下降;热胁迫下,黄瓜幼苗的可溶性蛋白含量下降,丙二醛(MDA)含量上升;最大光化学效率(Fv/Fm)、实际光化学量子产量(Yield)和光系统Ⅱ实际光化学效率(ΦSⅡ)都随着热胁迫温度的升高而降低,表观光合电子传递速率(ETR)在38℃高温下迅速上升,42℃高温下则急剧下降。相关性分析表明,电导率和Yield变化值与热害指数呈极显著性相关,SOD、APX、可溶性蛋白以及ETR的变化值与热害指数呈显著性相关。鉴于指标的简单可操作性,认为电导率、Yield和ETR变化值可以作为快速鉴定黄瓜幼苗耐热性的可靠指标。
Investigating the effects of high temperature on cucumber growth and sorting simple and reliable identification indexes of hot resistance have important roles in improving hot-resistant breeding efficiency.Physiological indexes and chlorophyll fluorescence parameters of 5 cucumber inbred lines treated at 38 ℃ and 42 ℃ hot stress conditions were determined.The results showed that the inbred lines with higher hot injury index have higher membrane permeability.As hot stress increase,SOD,APX activities and MDA levels elevates,but POD,CAT activities and soluble protein decreases The photochemical maximum efficiency of PSⅡ（Fv/Fm）,actual photochemical yield（Yield）,and actual photochemical efficiency of PSⅡ（ФPSⅡ）increase with hot stress rising.Electron transport rate（ETR）rise rapidly at 38 ℃ and decreased quickly at 42 ℃.There is an extremely significant correlation between electric conductivity percentage and hot injury index.The changes of SOD,APX,soluble protein content and ETR had significant correlation with hot injury index,too.The electric conductivity percentage,yield and ETR change could be reliable indexes for identifying hot resistance of cucumber seedlings according to simplicity and operability.
出处
《上海交通大学学报（农业科学版）》
2011年第5期61-66,共6页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科技兴农重点攻关项目[(沪农科攻字(2009)第2-1号]
关键词
黄瓜
热胁迫
生理反应
叶绿素荧光特性
cucumber
hot stress
physiological reaction
chlorophyll fluorescence characteristics
