摘要
目的探讨白介素18（IL-18）及IL-18结合蛋白（IL-18BP）在再生障碍性贫血（AA）患者血清中的表达及其临床意义。方法采用ELISA法检测29例AA患者和22名正常人血清IL-18和IL-18BP水平；实时定量RT—PCR方法检测IL-18及IL-18BP的mRNA表达。结果AA患者血清IL-18水平为（365．5±160．6）pg／ml，较正常对照组[（175．9±92．8）pg／ml]明显升高（P〈0．01）；重型AA患者的IL-18水平[（441．3±116．9）pg／ml]较慢性AA患者的IL-18水平[（326．4±167．0）pg／ml]升高更为明显（P〈0．05）；AA患者血清IL-18BP[（1788．6±523．8）pg／ml]较正常对照组[（1083．6±489．6）pg／ml]明显升高（P〈0．05），IL-18／IL-18BP比值在AA患者明显升高。RT—PCR结果证实AA患者IL-18和IL-18BP较正常对照组均明显升高，IL-18／IL-18BP比值明显升高。结论IL-18及IL-18BP在AA患者的免疫紊乱和发生、发展中起着一定的作用，重塑IL-18／IL-18BP平衡可能是AA治疗一个新的策略和方向。
Objective To investigate the role of IL-18 and IL-18BP balance in aplastic anemia （AA）. Methods A total of 29 AA patients and 22 controls were recruited in present research. The expressions of IL-18 and IL-18BP were measured by enzyme-linked immunosorbent assay （ELISA）. The mRNA expressions of IL-18 and IL-18BP were measured in all subjects using real-time quantitative polymerase chain reaction （RT-PCR）. Results The levels of the IL-18 in plasma of AA and normals were （365.5 ± 160.6 ） pg/ml and （175.9±92.8）pg/ml（P 〈0. 01 ）;and the expression of IL-18 in severe AA patients （441.3 ± 116.9） pg/ml were higher than that in non-severe AA patients （326.4 ± 167. 0） pg/ml （P 〈 0.05）. The level of IL-18BP was increased in plasma of AA （ 1788.6 ± 523.8 ） pg/ml than in normals （ 1083.6 ± 489.6 ） pg/ml（P 〈0.05）. But the ratio of IL-18/IL-18BP in AA patients was much higher than that in controls （P 〈0.05 ）. RT-PCR revealed the levels of IL-18 and IL-18BP mRNA were up-regulated in AA patients when compared to controls, but the ratio of IL-18/IL-18BP was significantly elevated in AA patients. Conclusion IL-18/IL-18BP imbalance may play an important role in pathogenesis of AA and regulating the balance of IL-18 and IL-18BP may be a therapeutic approach to AA.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2011年第11期783-785,共3页
Chinese Journal of Hematology
基金
基金项目：山东省自然科学基金（ZR2010HQ002）,山东大学自主创新基金（2009TS053）
