摘要
以绥农14为材料,利用框栽、砂培和15 N标记的方法,研究了大豆硝酸还原酶(NR)活性及其与施氮水平的关系。结果表明:框栽试验中大豆叶片NR活性随生育进程的推进大体上呈单峰曲线变化,营养生长阶段叶片NR活性较低,进入生殖生长期后逐渐升高,并于盛花期前后达到峰值,随后呈逐渐下降趋势;施氮水平对NR活性有明显影响,营养生长期表现为N135>N90>N45>N0,进入生殖生长期后没有表现出明显规律性;不同叶位叶片NR活性均表现为上部叶>中部叶>下部叶,且各叶位间差异呈极显著水平。砂培试验中,在大豆鼓粒始期(R5)至鼓粒盛期(R6)期间淋洗以15N标记的KNO3为氮源的营养液培养15d,大豆上、中、下部分各器官15N丰度表现为上部>中部>下部,表明大豆上部叶片硝酸还原酶活性高,促进了硝态氮的同化。
Field experiments were conducted to study the nitrate reductase activity and its relationship to nitrogen by using frame tests（pot without bottom）,sand culture and15N-urea at transplanting in soybean variety Suinong 14.Results showed that the activity of nitrate reductase in leaf changed as a signal peak curve with the soybean growth,lower in vegetative growth phase,higher in reproductive growth period and reached the peak in blooming period,then decreased gradually.Nitrogen application showed obvious effect on the nitrate reductase activity.The activities of nitrate reductase in leaves followed the order of N135N90N45N0 in vegetative growth stage,no clear regularity was found during the whole reproductive growth period.The activities of nitrate reductase in leaves were accorded with the order of upper leavesmid leaveslower leaves,and it was very significant differences（P0.01） on different node position.The result of sand experiment conducted with15N labeling method during beginning seed stage and full seed stage shown that15N abundance in various organs at different node position also followed the same order,suggesting that high level of nitrate reductase activity at upper leaves of soybean promoted the assimilation of NO-3.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第5期1036-1041,共6页
Journal of Nuclear Agricultural Sciences
基金
黑龙江省科技攻关项目(GA09B103
GA09B104)
黑龙江省博士后基金(LRB06-480)
关键词
大豆
硝酸还原酶
施氮水平
soybean
nitrate reductase
nitrogen applied level
