摘要
为研究互花米草对高硫环境的适应机制及其在入侵过程中的潜在意义,比较了互花米草和本土盐生植物芦苇在硫胁迫下抗氧化系统的响应差异.实验中两种植物均能在0～400mmol.L-1的Na2SO4处理下存活,其中互花米草过氧化氢(H2O2)水平在中高浓度处理下有所增加,期间超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性及花青素含量上升显著,过氧化物酶(POD)活性及抗坏血酸(ASA)含量变化不大,仅过氧化氢酶(CAT)活性呈轻微下降趋势.芦苇H2O2水平在最低处理浓度下即出现波动,但各处理组与对照差异未达到统计显著性,其ASA和花青素含量在中高处理浓度下上升显著,但所有抗氧化酶作用不明显.实验中200mmol.L-1及以上Na2S处理导致互花米草死亡,非致死浓度处理下,H2O2水平上升显著,所测SOD、POD、CAT和APX 4种酶活性也都有上升过程.100mmol.L-1及以上Na2S处理导致芦苇死亡,非致死浓度处理下,H2O2水平也出现上升过程,但只有SOD和CAT活性出现上升.Na2S处理下,两种植物的ASA和花青素作用均不明显.由此可见,互花米草较芦苇对硫复合物的响应差异主要体现于Na2S,前者对高硫环境的耐受性与其自身相对高效的抗氧化能力有关.
To explore the mechanism that Spartina alterniflora acclimates under high sulfur environment and its potential value in invasion process,the responses of antioxidant systems to sulfur stress were compared between the invasive S.alterniflora and the native Phragmites australis.Both species survived under Na2SO4 stress with concentrations 0—400mmol·L-1.S.alterniflora got increased H2O2 contents when exposed to higher levels Na2SO4,while its superoxide dismutase（SOD） and ascorbate peroxidase（APX） activities together with anthocyanin content increased as well.Catalase（CAT） activity of S.alterniflora decreased,but no significant changes were found on peroxidase（POD） and ascorbic acid（ASA）.P.australis got fluctuated H2O2 contents even under the lowest level Na2SO4,only such fluctuations were not statistically significant.ASA and anthocyanin contents increased in P.australis when exposed to higher levels Na2SO4,but no antioxidant enzymes were found functioning.S.alterniflora could not survive under Na2S stress with concentrations 200mmol·L-1 and above.Under lower levels Na2S,H2O2 contents together with activities of all the antioxidant enzymes,SOD,POD,CAT and APX,were found increased.P.australis could not survive under Na2S stress with concentrations 100mmol·L-1 and above.Under lower levels Na2S,H2O2 contents also increased,while only activities of SOD and CAT increased as well.ASA and anthocyanin in both species took little function under Na2S stress.In general,S.alterniflora and P.australis exhibited significant difference when exposed to sulfide stress,the better tolerance of S.alterniflora towards high sulfur environment was correlated with its effective antioxidant ability.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2011年第5期653-661,共9页
Journal of Fudan University：Natural Science
基金
国家自然科学基金资助项目(30970458
30770358)
中央高校基本科研业务费专项资金(1105020807)
关键词
生物入侵
互花米草
芦苇
抗氧化系统
硫
盐沼
bioinvasion
Spartina alterniflora
Phragmites australis
antioxidant system
sulfur
saltmarsh
