摘要
目的探讨罗布麻提取物对血管紧张素II(Angiotensin Ⅱ,Ang Ⅱ)诱导的大鼠心肌纤维化细胞的影响及其可能作用机制。方法采用Ang Ⅱ诱导心肌成纤维细胞复制心肌纤维化模型,细胞分组为空白对照组(C组)、模型组(Ang Ⅱ组)、罗布麻提取物高剂量组(0.8mg.L-1,Ang Ⅱ+AVE1)、罗布麻提取物中剂量组(0.4mg.L-1,Ang Ⅱ+AVE1)、罗布麻提取物低剂量组(0.2mg.L-1,Ang Ⅱ+AVE1)及阳性药对照缬沙坦组(10-6mg.L-1,Ang Ⅱ+VL),给药48h后,通过MTT检测心肌成纤维细胞增殖情况,ELISA法及RT-PCR法检细胞上清及细胞中Ⅰ、Ⅲ型胶原、TGF-β(Transforming growthfactor-β)蛋白及mRNA的表达。结果在Ang Ⅱ诱导的心肌纤维化模型中,罗布麻提取物可抑制ECM(Extracellularmatrix)胶原成分(Col Ⅰ和Col Ⅲ)的积聚;TGF-β在心肌纤维化大鼠心肌中表达显著增加,罗布麻提取物可降低TGF-βmRNA及蛋白的表达。结论罗布麻提取物可通过对降低TGF-β的表达延缓心肌纤维化的发生发展。
Objective To investigate the influence and the mechanisms involved in the regression of apocynum venetum extract（AVE）on Ang Ⅱ-induced myocardial fibrosis.AVE on myocardial fibrosis.Methods Rat cardiac fibroblasts were pretreated with Ang Ⅱ,and then,treated with different concentrations of AVE（0.8mol·L-1、0.4mol·L-1、0.2mol·L-1）.The effect of AVE on the proliferation of cardiac fibroblast stimulated by Ang Ⅱ was detected by MTT,while the expression of Col Ⅰ/Col Ⅲ and TGF-β by ELISA and RT-PCR.Results In the cardiac fibroblast,the over expression of Col Ⅰ and Col Ⅲ,induced by Ang II,could be inhibited by AVE.What＇s more,compared with C group,TGF-β was increased obviously in the Ang Ⅱ group,and all the AVE groups were able to inhibit the increament of TGF-β stimulated by AngII.Conclusion AVE can reduce the expression of mRNA and protein of TGF-β,and as a result,lighten myocardial fibrosis
出处
《中国热带医学》
CAS
2011年第9期1113-1115,共3页
China Tropical Medicine
基金
2008年国家自然科学基金(No.30872194)
