摘要
Background Murine cytomegalovirus （MCMV） early protein Ml12-113 is involved in viral DNA replication and believed to play a crucial role in the viral pathogenesis. To investigate the biological function of Ml12-113 protein in the pathogenesis of the brain disorders caused by cytomegalovirus （CMV）, a screening for proteins interacting with Ml12-113 was performed by a yeast two-hybrid system. Methods Bait plasmid pGBKT7-M112-113 was constructed and transformed into AH109 yeast. After confirmation of the expression of MCMV Ml12-113 in yeast, the bait yeast was mated with a prey yeast containing mouse brain cDNA library plasmid to screen the proteins interacting with M 112-113. Interactions between Ml12-113 and the obtained proteins were verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion. Results Two proteins interacting with M112-113 were identified, including metastasis-associated 1 （MTA1） and zinc finger, CCHC domain containing 18 （ZCCHC18）. Ml12-113 protein could interact with MTAt or ZCCHC18 in yeast and mammalian cells. Conclusion The interactions of Ml12-113 with MTA1 or ZCCHC18 may be related to the pathogenesis of MCMV-associated disease in central nervous system.
Background Murine cytomegalovirus （MCMV） early protein Ml12-113 is involved in viral DNA replication and believed to play a crucial role in the viral pathogenesis. To investigate the biological function of Ml12-113 protein in the pathogenesis of the brain disorders caused by cytomegalovirus （CMV）, a screening for proteins interacting with Ml12-113 was performed by a yeast two-hybrid system. Methods Bait plasmid pGBKT7-M112-113 was constructed and transformed into AH109 yeast. After confirmation of the expression of MCMV Ml12-113 in yeast, the bait yeast was mated with a prey yeast containing mouse brain cDNA library plasmid to screen the proteins interacting with M 112-113. Interactions between Ml12-113 and the obtained proteins were verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion. Results Two proteins interacting with M112-113 were identified, including metastasis-associated 1 （MTA1） and zinc finger, CCHC domain containing 18 （ZCCHC18）. Ml12-113 protein could interact with MTAt or ZCCHC18 in yeast and mammalian cells. Conclusion The interactions of Ml12-113 with MTA1 or ZCCHC18 may be related to the pathogenesis of MCMV-associated disease in central nervous system.
基金
This study was supported by a grant from National Natural Science Foundation of China （No. 30671859）.
