摘要
通过稀释法对从赣南柑橘园采集的土样进行微生物分离,获得细菌、真菌和放线菌共52株,其中真菌3株、细菌27株、放线菌22株。进一步通过皿内实验筛选到1株对柑橘炭疽病菌和溃疡病菌有较强抑制作用、且效果稳定的放线菌株ML27,对其进行抑菌活性测定和抑菌机理初探。结果表明,该菌株对炭疽病菌的抑制率为72.15%,对溃疡病菌的抑菌圈为2.05 cm;其抑菌机理主要是对真菌的细胞壁产生影响,对细菌的菌体具有溶解作用。
The microorganisms were isolated from soil from orangery in Southern Jiangxi Province by dilution and 52 strains including 3 fungus,27 bacteria and 22 actinomyces were obtained.An actinomyce strain ML27 exhibited high inhibitory effect on the growth of Colletotrichum gloeosporioides and Xanthomonas axonopodis pv.citri in vitro,the inhibitory effect and mechanisms of which were investigated primarily.The results indicated that strain ML27 inhibited the growth of Colletotrichum gloeosporioides by 72.15% and the growth of Xanthomonas axonopodis pv.citri decreased to 2.05 cm compared to the uninoculated control in vitro.The mechanisms of inhibition to pathogen lay mainly in disturbance to the cell wall of fungi and dissolution of the body of bacteria.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2011年第5期889-892,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省自然科学基金(2010GQN0056)
江西省教育厅青年科学基金项目(GJJ11085)
江西农业大学博士启动基金和江西农业大学校科技基金
关键词
放线菌
柑橘炭疽病菌
柑橘溃疡病菌
拮抗作用
actinomyces
Colletotrichum gloeosporioides
Xanthomonas axonopodis pv.citri
antagonistic action
