摘要
以狼尾蕨无菌苗的叶片为外植体,探讨不同类型的细胞分裂素对不定芽诱导、增殖和成苗的影响。结果显示:在附加1.0 mg/L和2.0 mg/L KT或1.0 mg/L和2.0 mg/L CPPU的诱导培养基上叶片诱导产生叶状体状不定芽,转到添加0.5 mg/L KT或0.5 mg/L CPPU的增殖培养基上可形成叶芽状不定芽;在附加1.0 mg/L和2.0mg/L BAP的培养基上叶片诱导可产生叶状体状不定芽,转到添加0.5 mg/L BAP的增殖培养基上不定芽仍保持叶状体状。叶芽状不定芽在附加0.5 mg/L CPPU的增殖培养基上连续继代培养,每代芽增殖系数达5以上。叶芽状不定芽转到附加0.2 mg/L IBA的成苗和生根培养基上,能发育成具有根、茎、叶的完整小植株,成苗率达100%。
The leaves of sterile plantlet of Davallia bullata was used as explant to study the effect of different types of cytokinin on adventitious bud induction,proliferation and shooting.The thallus-like adventitious bud was induced from the leaf in the modified MS medium with 1.0mg/L KT,or 2.0 mg/L KT,or 1.0 mg/L CPPU,or 2.0mg/L CPPU,and formed leafbud-like adventitious bud in the bud proliferative medium with 0.5 mg/L KT or 0.5 mg/L CPPU.The thallus-like adventitious bud was induced as well from the leaf in the induction medium with 1.0 mg/L or 2.0 mg/L BAP and stayed the same shape in proliferation medium with 0.5 mg/L BAP.In the bud proliferative medium with 0.5 mg/L CPPU,the coefficient of bud propagation was above 5.The leafbud was 100%,and developed into intact regenerated plantlet with root,stem and leaf in the MS medium with 0.2 mg/L IBA.
出处
《江苏农业学报》
CSCD
北大核心
2011年第5期1100-1103,共4页
Jiangsu Journal of Agricultural Sciences
基金
江苏省科技厅科技支撑项目(BE2010339)
江苏省农业综合开发科技推广项目(2010KJ-53)
江苏省农业科技自主创新基金项目[CX(10)320]
关键词
狼尾蕨
叶片
细胞分裂素
不定芽
植株再生
Davallia bullata.
leaf
cytokinin
adventitious bud
plant regeneration
