摘要
建立HPLC法同时测定来自中国不同地区的地黄中次黄嘌呤、尿苷、腺嘌呤、鸟苷、腺苷等5种核苷类成分的含量。采用的色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.04 mol.L-1磷酸二氢钾溶液梯度洗脱,流速1 mL.min-1,柱温30℃,检测波长254 nm。次黄嘌呤、尿苷、腺嘌呤、鸟苷和腺苷的质量浓度分别在1.0～16.0μg.mL-1(r2=0.999 8),5.0～80.0μg.mL-1(r2=0.999 8),1.0～16.0μg.mL-1(r2=0.999 5),1.25～20.0μg.mL-1(r2=0.999 8)和1.0～16.0μg.mL-1(r2=0.999 8)内线性关系良好,平均回收率为98.8%～100.7%。结果表明,不同地区的地黄中次黄嘌呤、尿苷、腺嘌呤、鸟苷和腺苷的含量有显著性差异。该方法准确,重复性好,适用于地黄药材中5种核苷类成分的含量测定。
This study is to establish a method for simultaneously determination of five nucleosides and nucleobases,including hypoxanthine,uridine,adenine,guanosine and adenosine in Rehmannia glutinosa Libosch.which was collected from different regions in China.A Diamonsil C18 column（250 mm × 4.6 mm,5 μm） was used.Acetonitrile and 0.04 mol.L-1 potassium dihydrogen phosphate solution were adopted as mobile phase with gradient elution.The flow rate was 1 mL.min-1 and column temperature was 30 ℃.The detection wavelength was at 254 nm.The method had good linearity over the range of 1.0 - 16.0 μg.mL-1（r2 = 0.999 8）,5.0 - 80.0 μg.mL-1（r2 = 0.999 8）,1.0 - 16.0 μg.mL-1（r2 = 0.999 5）,1.25- 20.0 μg.mL-1（r2 = 0.999 8） and 1.0 -16.0 μg.mL-1（r2 = 0.999 8） for hypoxanthine,uridine,adenine,guanosine and adenosine,respectively.The average recoveries were between 98.8% and 100.7%.The content of hypoxanthine,uridine,adenine,guanosine and adenosine in Rehmannia glutinosa Libosch.from different regions was significantly different.This established method was sensitive and reliable for the quantification of five chemical constituents in Rehmannia glutinosa Libosch.
出处
《药学学报》
CAS
CSCD
北大核心
2011年第11期1380-1384,共5页
Acta Pharmaceutica Sinica
基金
Project supported by Shanghai Leading Academic Discipline Project(Y0302)
E-institute of Shanghai Municipal Education Commission(E03008)
Innovative Research Team in Universities,Shanghai Municipal Education Commission
National Science and Technology Major Project(2009ZX09311-003)
关键词
高效液相色谱
地黄
核苷
碱基
同时测定
high performance liquid chromatography
Rehmannia glutinosa Libosch.
nucleoside
nucleobase
simultaneous determination
