摘要
利用食肉目线粒体Cyt b基因的特异性引物对2010年4月在广东车八岭自然保护区内发现的爪鞘进行了分子鉴定。所测序列与亚洲黑熊(Ursus thibetanus)的同源性最高,达97.2%~99.1%,确定爪鞘来自于亚洲黑熊,为近20 a来首次在车八岭保护区发现的亚洲黑熊存在痕迹。
DNA was direcdy extracted from a claw sheath found in Chebaling National Nature Reserve, then the mtDNA Cyt b gene fragment was amplified and sequenced. Sequences were aligned with the GenBank by BLAST software, which showed the highest homology to the sequences from the black bear ( Ursus thibetanus) , reaching 97.2%-99. 1%. The claw sheath was identified from U. thibetanus. It is the first time we have discovered trace of U. thibetanus appeared in Chebaling National Nature Reserve in recent 20 years.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2011年第11期105-107,共3页
Journal of Northeast Forestry University