摘要
目的研究邓老凉茶颗粒的质量控制方法。方法采用HPLC法建立了邓老凉茶颗粒甲醇提取液的HPLC指纹图谱,收集了不同批次的11批产品进行测定,并使用主成分分析法对指纹图谱进行了模式识别研究。结果 11批邓老凉茶颗粒指纹图谱相似度达到0.95以上;经主成分分析,16个共有峰可综合为3个主成分,其累积贡献率达91.89%,异绿原酸B的相对峰面积为邓老凉茶颗粒指纹图谱中影响最大的指标。结论该方法稳定可靠,可以有效地用于邓老凉茶颗粒的质量评价和质量控制。
Objective To establish a method for the quality control of Deng＇s Herbal Tea Granula. Methods An HPLC method was developed to establish the fingerprint of Deng＇s Herbal Tea Granula methanol extract and 11 samples from various batches were analyzed. Furthermore, principal component analysis （PCA） was used to investigate pattern recognition. Results The similarity among 11 batches of samples was no less than 0.95. The PCA showed that 16 common peaks were integrated into three principal components and their accumulation contributing rate amounted to 91.89%. The relative peak area of isochlorogenic acid B was the most effective index in fingerprint of Deng＇s Herbal Tea Granula. Conclusion This method is available for the quality evaluation and quality control of Deng＇s Herbal Tea Granula.
出处
《中草药》
CAS
CSCD
北大核心
2011年第11期2231-2234,共4页
Chinese Traditional and Herbal Drugs
基金
广东省科技计划重点项目(2010A030100013)
关键词
邓老凉茶颗粒
指纹图谱
主成分分析
质量控制
异绿原酸B
HPLC
Deng＇s Herbal Tea Granula
fingerprint
principal component analysis （PCA）
quality control
isochlorogenic acid B
HPLC
