摘要
本研究旨在探讨螺旋酶抗原基因(HAGE)在急性髓系白血病(AML)和慢性髓系白血病(CML)中的表达状况和临床意义。应用实时定量PCR(RQ-PCR)方法检测AML和CML患者骨髓单个核细胞中HAGE cDNA的表达。结果表明:74例AML患者中11例(14.8%)存在HAGE过表达(117.12%-9842.70%,中位434.96%);HAGE过表达患者年龄显著高于HAGE阴性者(中位年龄分别为67和45岁,p=0.001)。HAGE表达在单核细胞亚型AML(M4和M5,7/20,35.0%)中明显高于其它亚型AML(4/54,7.4%)(p=0.007)。28例核型正常的AML患者中8例(28.6%)呈HAGE过表达,而40例核型异常的AML中仅3例(7.5%)存在HAGE表达(p=0.041)。9/26例(34.6%)CML患者存在HAGE过表达,加速期和急变期患者中HAGE表达率(4/4,100%)明显高于慢性期患者(5/22,22.7%)(p=0.008)。结论:HAGE cDNA表达与AML单核细胞亚型类别相关,且与CML疾病进展相关。
The aim of this study was to investigate the expression status of the helicase antigen（HAGE） transcript and its clinical significance in patients with acute myeloid leukemia（AML） and chronic myeloid leukemia（CML）.The expression of HAGE cDNA in bone marrow mononuclear cells from AML and CML patients was detected by using real-time quantitative PCR.The resuts indicated that overexpression of HAGE transcript（117.12%-9842.70%,median 434.96%） was detected in 14.8%（11/74） AML patients.AML patients with HAGE cDNA expression were significantly older than those HAGE-negative patients（median 67 and 45 years,respectively,p=0.001）.HAGE cDNA expression was more frequently present among the patients with acute monoblastic leukemia（M4 and M5,7 of 20,35.0%）,compared to the patients with acute non-monoblastic leukemia（M1,M2,M3 and M6,4 of 54,7.4%）（p=0.007）.28.6%（8/28） cases with normal karyotypes showed HAGE cDNA overexpression,significantly higher than 7.5%（3 of 40） in those with chromosomal abnormalities（p=0.041）.Overexpression of HAGE transcript was found in 9（34.6%） CML cases and more frequently observed at accelerated phase and blast crisis（4/4,100%） than that at chronic phase（5/22,22.7%）（p=0.008）.It is concluded that HAGE cDNA expression is relevant to specific subtypes of AML and to the progression of CML.
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第5期1171-1175,共5页
Journal of Experimental Hematology
