摘要
目的探讨神经营养因子在肺腺癌细胞株A549中的表达及临床意义.方法体外培养肺腺癌A549细胞株,观察细胞形态变化.取代培养后3 d的A549细胞,用免疫组化检测神经营养因子GDNF、BD-NF、NT3和NT4在肺腺癌细胞株A549的表达.结果神经营养因子GDNF、BDNF、NT3和NT4在部分肺腺癌细胞株A549中表达.结论神经营养因子GDNF、BDNF、NT3和NT4可能在肺癌的增殖中发挥作用.
Objective To investigate the expression of neurotrophic factors（GDNF,BDNF,NT3,NT4）in human lung adenocarcinoma cell lines A549 and explore the functional significance.Method After cell lines A549 were cultured and morphological changes were observed,the expression of neurotrophic factors GDNF,BDNF,NT3 and NT4 were detected by immunohistochemistry in cell lines A549 on 3 days.Result Neurotrophic factors GDNF,BDNF,NT3and NT4 were expressed in part of cell lines A549.Conclusion The neurotrophic factors GDNF,BDNF,NT3 and NT4 may play a role in the proliferation of lung cancer cells.
出处
《昆明医学院学报》
2011年第8期46-49,共4页
Journal of Kunming Medical College
关键词
A549
神经营养因子
肺腺癌
A549
Neurotrophic factors
Lung adenocarcinoma
