摘要
为了能够快速检测牛奶质量问题,采用3维荧光光谱和多峰拟合成分的方法,对纯牛奶的3维荧光光谱的特征及其主要成分进行了分析研究。结果表明,激发光谱在250nm～310nm范围,最佳激发波长在290nm附近;发射光谱主要在285nm～500nm,具有较强的荧光,荧光峰在330nm附近。液态纯牛奶的荧光光谱主要由5个谱峰(Ⅰ～Ⅴ)组成,其中峰Ⅰ是乳糖影响下部分蛋白质的荧光、峰Ⅱ为脂肪和VE共同产生的、峰Ⅲ为蛋白质直接所为、峰Ⅳ是其它成分影响下的蛋白质的荧光、峰V是VA,VB_1和VB_6等微量元素共同产生的。研究结果可为液态纯牛奶的荧光光谱检测技术研究提供实验和理论依据。
To detect the quality of pure milk rapidly, its spectral characteristics were analyzed combining 3-D fluorescent spectra with multi-peak fitting method, and its main compositions were obtained. The results show that its excitation spectrum ranges from 250nm to 310nm, and the peak wavelength is 290nm. The main emission spectrum ranges from 280nm to 500nm with the peak adjacent to 330nm. Meanwhile, the fluorescent spectrum has five spectral peaks（ Ⅰ-Ⅴ ）, where peak Ⅰis generated by some proteins under the influence of lactose, peak Ⅱis generated by fat and vitamin E jointly, peak Ⅲis generated by protein directly, peak Ⅳis generated by some proteins under some unknown influence, and peak V is generated jointly by microclements including vitamin A, vitamin B1, and B6. The study results provide some basic principles for studying milk composition with fluorescent spectroscopy.
出处
《激光技术》
CAS
CSCD
北大核心
2011年第6期742-744,769,共4页
Laser Technology
基金
江苏省自然科学基金资助项目l(BK2009066)
江苏省教育厅资助项目(JH08-18
CX08B-088Z)
关键词
光谱学
3维荧光光谱
多峰拟合
液态纯牛奶
成分
spectroscopy
3-D fluorescent spectra
multi-peak fitting
pure liquid milk
composition
