摘要
本文综述了β-葡聚糖酶活力的测定方法,如黏度法、生色底物法、琼脂糖扩散法、酶联免疫吸附分析法(ELISA)和目前使用较多的还原端基法,如3,5-二硝基水杨酸(DNS)法、Somogyi-Nelson法、二金鸡纳酸(BCA)法和最新涌现的用于微量测定的3-甲基-2-苯并噻唑酮腙(MBTH)法,并就还原端基法介绍了其具体实验手段,如试管法、罐法、微孔板法、流动注射法(FIA)。分析了方法中存在的问题并展望了β-葡聚糖酶活力测定方法研究的发展趋势,如安培法和等温滴定量热法等。
In this paper,the present determination methods for β-glucanase activity are reviewed,which includes viscometric method,chromomeric method,agar diffusion method,and commonly used end-reducing methods such as DNS,Somogyi-Nelson and BCA methods,as well as novel MBTH method.In addition,the end-reducing methods are classified as end-point method,multiple-point method,microplate method,and flow injection analytic method according to the operation procedures.Meanwhile,current problems with these methods are discussed,and the future development trends of the determination methods are proposed.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第19期316-320,共5页
Food Science
关键词
Β-葡聚糖酶
测定
DNS法
MBTH法
微孔板法
流动注射法
试管法
罐法
β-glucanase
determination
DNS method
MBTH method
microplate method
flow injection method
end point method
multiple pointspoints method
