摘要
目的:建立RP-HPLC法测定八维钙锌片中维生素D2和维生素E含量的方法。方法:采用Phenomenex-C18柱(4.6 mm×150 mm,5μm);以为甲醇流动相;紫外检测波长为265 nm;流速为1 mL.min-1。结果:维生素D2的线性范围为0.8473～4.2365μg.mL-1(r=0.9996),平均回收率为99.7%,RSD%为1.82%(n=9);维生素E的线性范围为0.1625～0.8125mg.mL-1(r=0.9996),平均回收率为100.3%,RSD%为1.93%(n=9)。结论:本方法操作简便,结果准确、可靠,可同时测定八维钙锌片中维生素D2和维生素E的含量。
Objective： To establish the method for the determination vitamin D2 and vitamin E in compound reserpine tablets by RP -HPLC. Method： The separation was performed Phenomenex CIs. methanol as the mobile phase with the flow rate of 1 mL · min^- 1, and 265 nm as the detective length. Results： All the results for the standards curve, the average recovery and the separation showed that the method is satisfactory. Conclusion：The method is simple, fast and accurate for analysis of Baweigaixin tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第11期2180-2181,共2页
Chinese Journal of Pharmaceutical Analysis
