摘要
目的观察唑来膦酸(ZOL)单药在体内对人骨肉瘤裸鼠移植瘤的治疗作用,明确ZOL联合DOX化疗治疗骨肉瘤的疗效。方法人骨肉瘤细胞株OS-732复苏后体外培养传代备用。48只SPF级BALB/c雌性裸鼠备用。运用组织块接种法于裸鼠右腋后皮下成瘤,实验分为6组:生理盐水对照组(NS组),DOX组,ZOL低、中、高剂量组,DOX+ZOL组。根据肿瘤倍增体积和瘤重得出抑瘤率,按照金氏公式计算Q值明确两种药物对抑制肿瘤的相互作用关系。免疫组化Ⅷ因子评价肿瘤内微血管密度(MVD)、Tunel凋亡细胞计数。结果 DOX组、ZOL低剂量组、ZOL中剂量组、ZOL高剂量组、DOX+ZOL组抑瘤率分别为43.01%、16.83%、23.88%、6.66%、49.55%,Q值为0.87,显示二者效应为单纯相加;DOX组、DOX+ZOL组凋亡指数、MVD与NS组有统计学差异,但DOX组与DOX+ZOL组间无差异。结论对于人骨肉瘤OS-732皮下移植裸鼠模型,ZOL单药干预抑瘤作用不显著;ZOL与DOX联合用药在本研究实验动物体内表现为相加作用。
Objective To identify the combined effects of zoledronic acid （ZOL） with doxorubicin （DOX） against hu- man osteosarcoma xenograft model in nude mice. To evaluate the efficacy of conrbination of ZOL with DOX. Methods Es- tablishment of human osteosarcoma 0S-732 xenograft model in nude mice. 48 nude mice were divided into 6 groups： NS, DOX, ZOL （low）, ZOL （median）, ZOL （high）, DOX ＋ ZOL. To calculate the inhibition rate according to the tumor vol- ume and weight. And then to evaluate the interaction between two drugs by Q values from Jin＇ s formula. The tumors were ob- served immunohistochemical staining for VIH factor, mlcro-vessel density （MVD）, TUNEL apoptosis index （AI）. Results The inhibition rate of tumor weight of DOX, ZOL （low, median, high）, DOX ＋ ZOL groups were 43.01%, 16.83%, 23.88%, 6.66%, 49.55% respectively. The Q value was 0.87, which showed an additional effect between DOX and ZOL. There was a statistical significance between NS and DOX, DOX ＋ ZOL at MVD and AI statistical analysis, but no significance between DOX and DOX ＋ ZOL. Conclusions ZOL has no capability to inhibit proliferation of human osteosarcoma xenograft model in nude mice. DOX and ZOL play an additional effect in this experiment.
出处
《山东医药》
CAS
北大核心
2011年第40期22-24,F0003,共4页
Shandong Medical Journal
基金
首都医学发展科研基金资助项目(2005-1009)
