摘要
目的探讨乌司他丁(Ulinastatin,UTI)单独和联合泰索帝(Taxotere,TXT)对人原代乳腺癌细胞增殖、侵袭、凋亡和环氧合酶-2(Cyclooxygenase-2,COX-2)、前列腺素E-2(Prostaglandin E-2,PGE-2)、白介素-10(Interleukin-10,IL-10)表达的影响。方法采用酶消化法提取人乳腺癌组织中的癌细胞,进行原代培养,将细胞随机分为4组:空白对照组、UTI组(800 IU/ml)、TXT组(3.7μg/ml)、UTI+TXT组,采用MTT法、Transwell小室试验和流式细胞术分别检测细胞的增殖、侵袭和凋亡;半定量RT-PCR和Western blot检测细胞COX-2、IL-10基因和蛋白水平的表达;ELISA检测细胞PGE-2的分泌。结果与对照组相比,UTI和TXT均能抑制乳腺癌细胞的增殖和侵袭,并促进凋亡,UTI与TXT联合作用更显著;UTI、TXT单独和联合处理后,细胞COX-2、IL-10的表达均较对照组明显下降,并有效降低了PGE-2的分泌水平。结论 UTI和TXT均能抑制乳腺癌细胞的体外生长,UTI可增强TXT的抑制作用,其机制可能与UTI降低COX-2、PGE-2、IL-10的水平有关。
Objective To investigate the effect of ulinastatin（UTI）,alone or combined with Taxotere（TXT）,on proliferation,invasion and apoptosis of primary human breast cancer cells as well as expressions of cyclooxygenase-2（COX-2）,prostaglandinE-2（PGE-2） and interleukin-10（IL-10）.Methods Human breast cancer cells were extracted by enzyme digestion and subjected to primary culture,then divided into blank control,UTI（800 IU / ml）,TXT（3.7 μg / ml） and UTI ＋ TXT group.The proliferation,invasion and apoptosis of cells were determined by MTT test,Transwell test and flow cytometry,while the expressions of COX-2 and IL-10 at gene and protein levels by semi-quantitative RT-PCR and Western blot,respectively,and the secretion of PGE-2 by ELISA.Results Both UTI and TXT inhibited the proliferation and invasion while promoted the apoptosis of breast cancer cells as compared with control,while UTI ＋ TXT was more effective.After treatment with UTI,TXT and UTI ＋ TXT,the expression levels of COX-2 and IL-10 as well as secretion level of PGE-2 decreased significantly as compared with those in control group.Conclusion Both UTI and TXT inhibited the growth of breast cancer cells in vitro.However,UTI enhanced the inhibitory effect of TXT by a potential mechanism of decreasing COX-2,PGE-2 and IL-10 levels.
出处
《中国生物制品学杂志》
CAS
CSCD
2011年第10期1197-1202,共6页
Chinese Journal of Biologicals
基金
重庆市科技委员会科技攻关资助项目(CSTC
2008AC5082)
关键词
乌司他丁
泰索帝
乳腺肿瘤
环氧合酶-2
前列腺素E-2
白介素-10
Ulinastatin（UTI）
Taxotere（TXT）
Breast tumor
Cyclooxygenase-2（COX-2）
Prostaglandin E-2（PGE-2）
Interleukin-10（IL-10）
