摘要
目的比较慢性牙周炎患者和健康对照者非刺激性全唾液(WUS)中的差异表达蛋白。方法采集5名慢性牙周炎患者和5名健康者的WUS,利用双向凝胶电泳(2-DE)分离蛋白,将二者之间的差异蛋白质点进行基质辅助激光解析电离串联飞行时间质谱鉴定。结果通过软件对2组样品的双向胶上的点进行对比分析,发现17个差异点,经质谱鉴定明确了13个点的蛋白类型,其中737、725、692、986为同一种蛋白,716、535为相同蛋白,分别是血清白蛋白、β-纤维蛋白、重组N-叶apo型人血清转铁蛋白2型晶体B链、免疫球蛋白α-1链C区、人血清白蛋白GA复合物、Rca-Rhogdi复合物的B链、PRO2044、理论蛋白以及未命名蛋白,而且它们在慢性牙周炎患者全唾液中的表达均上调。结论比较慢性牙周炎患者和健康者WUS中的蛋白图谱时,发现至少9种与疾病相关的差异蛋白。这种蛋白组学技术也许能更好地理解慢性牙周炎的发病机制。
Objective To compare the proteomic profiles of whole unstimulated saliva（WUS） of subjects with chronic periodontitis（CP） with that of healthy volunteers,and to identify proteins.Methods WUS was obtained from five subjects with CP and five healthy subjects,and proteins were separated using two-dimensional gel electrophoresis（2-DE）. Proteins,the level of which was significantly different among the two groups,were identified by computer image analyses and subsequent matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry（MALDI-TOF-TOF MS）.Results Seventeen differentially expressed points in the 2-DE gel between the two samples were found by pro-fessional software.And protein types of thirteen points were then identified.Among them,the point 737,725,692,986 was the same protein,as well as the point 716,535.They were respectively serum albumin;β-fibrin;chain B,human serum transferrin,recombinant N-terminal lobe,Apo form,crystal form 2;Ig alpha-1 chain C region;the GA module complexed with human serum albumin（HAB-GA）;chain B,crystal structure of a Rca-Rhogdi complexes;PRO2044; hypothetical protein;unnamed protein.Compared with whole saliva of healthy control subjects,the levels of them were increased in whole unstimulated saliva of CP subjects.Conclusion Comparison of the proteomic profiles of WUS of CP subjects with that of healthy control subjects reveals at least 9 differential proteins.The approach might be helpful to better understand to the etiology of CP.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2011年第5期519-521,525,共4页
West China Journal of Stomatology
关键词
慢性牙周炎
非刺激性全唾液
差异蛋白
双向电泳
质谱
chronic periodontitis
whole unstimulated saliva
differentiated protein
two-dimensional gel electro-phoresis
mass spectrometry
