摘要
目的 研究孕期丰富环境和应激刺激对发育期仔鼠恐惧行为的影响.方法 将初次妊娠11天的孕鼠随机分为：应激组、丰富环境组和对照组,每组仔鼠分别于生后25d、45d和60d进行旷场实验和防御退缩实验测试.结果 （1）在旷场实验中,各日龄应激组仔鼠均较相应对照组中央格停留时间延长,总穿格数及站立次数减少（all P＜0.05）,与之相反,生后25d时丰富环境组仔鼠较对照组总穿格数增加[（101.75±11.87）格；（89.13±11.20）格；P＜0.05],而中央格停留时间缩短[（1.88±0.64）s;（3.63±1.41）s;P＜0.05].（2）在防御退缩试验中,生后25d时,应激组较对照组潜伏期延长（P＜0.05）；生后45 d时,不仅潜伏期延长（P＜0.05）,自小室退出次数亦减少（P＜0.05）；而生后60d时,在上述表现的基础上,给予束缚应激刺激后其在小室内的时间较相应对照组明显延长（P＜0.05）；与之相反,生后25d时,丰富环境组较对照组潜伏期缩短[（10.43±4.27）s; （22.16±4.50）s; P＜0.05],自小室退出次数增加[（6.34±1.03）次；（3.94±1.08）次；P＜0.05].结论 孕期给予丰富的环境可以降低其幼鼠的恐惧水平,增加其探究行为.
Objective To investigate the effect of different prenatal stimulation on fearful behavior of offspring.Methods On gestational days 11,primigravid female rats were randomly assigned to three groups：enriched group,stressed group and the control group.Only male offspring were used in the present study.Open field test and the defensive withdrawal test were performed at P25,P45 and P60.Results 1.Open-field test：PS offspring at any age had greater central grille time,decreased total cross number and stand number than their corresponding controls （all P 〈 0.05 ）.On the contrary,the prenatal enriched offspring at 25 days of age had increased total cross number （ 101.75 ± 11.87 ;89.13 ± 11.20 ; P 〈 0.05 ） and shorter central grille time （ （ 1.88 ± 0.64） s;（3.63 ± 1.41 ）s; P 〈 0.05） than their controls.2.Defensive-withdrawal test：when compare to the controls,PS offspring had greater latencies at P25,greater latencies and decreased number of exits from the chamber at P45,and greater latencies,decreased number of exits from the chamber and increased time in the chamber with restraint at P60 （ all P 〈 0.05 ）.On the contrary,the prenatal enriched offspring at 25 days of age had shorter latencies （ （ 10.43 ± 4.27 ） s; （ 22.16 ± 4.50） s; P 〈 0.05 ） and increased number of exits from the chamber than their controis （6.34 ± 1.03 ;3.94 ± 1.08 ; P 〈 0.05 ）.Conclusion Environmental enrichment during pregnancy only can decrease fearfulness and enhance exploratory behavior of their infancy offspring.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2011年第10期893-895,共3页
Chinese Journal of Behavioral Medicine and Brain Science
关键词
孕期应激
丰富环境
行为
旷场试验
Prenatal stress
Environmental enrichment
Behavior
Open-field test
