摘要
采用传统生理生化鉴定方法,16S rRNA基因序列分析技术,16S-23S rRNA间区序列多态性分析技术,变性梯度凝胶电泳技术(DGGE),对分离于自然发酵乳中的9株粪肠球菌和6株屎肠球菌进行鉴定,并对4种鉴定方法进行比较和评价。结果表明,16S-23S rRNA间区序列多态性分析技术和DGGE技术不但可以快速、精确地区分粪肠球菌和屎肠球菌,而且能够将粪肠球菌和屎肠球菌种内的不同基因亚型区分开,而传统生理生化鉴定方法和16S rRNA基因序列分析技术较以上两种方法区分效果略差。
In order to accurately identify Enterococcus faecalis and Enterococcus faecium isolated from natural fermented dairy products, in this study, four identification methods, namely traditional physiological and biochemical identification methods, 16S rRNA gene analysis, 16S -23S rRNA Intergenic spacer region polymorphism and Denaturing Gradient Gel Electrophoresis were carried out to differentiate the tested strains. Meanwhile, the identification of these four methods was compared with evaluated ability. The results showed that except for the methods of the traditional physiological and biochemical identification methods and 16S rRNA gene analysis, 16S- 23S rRNA Intergenic spacer region polymorphism and Denaturing Gradient Gel Electrophoresis not only were competent, but also could divide Enterococcus faecalis and Enterococcus faecium into different kinds of gene isoforms.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第9期98-103,共6页
Food and Fermentation Industries
基金
内蒙古自然科学基金重点项目(No.2010zd15)
国家科技支撑计划项目(No.2009BADC1B01)
内蒙古自然基金(No.2009MS0409)
关键词
粪肠球菌
屎肠球菌
分类鉴定
基因亚型
Enterococcus faecalis, Enterococcus faecium, differentiation, gene isoforms
