摘要
本试验采用Trizol Reagent提取拟南芥总RNA,设计相关引物,采用反转录PCR方法克隆TGA2转录因子;利用酶切连接方法,将该基因正向导入蛋白表达载体。经过相关检验,TGA2转录因子连接到pMXB10载体内含肽(Intein)的N端,成功构建包含TGA2转录因子的pMXB10-TGA2蛋白表达载体。
After extracting the Arabidopsis thaliana RNA with trizol reagent and designing primes,TGA2 transcription factor gene was cloned by RT-PCR.The experiment connects the TGA2 transcription factor with intein＇s N-Terminal of pMXB10 vectors.The TGA2 transcription factor gene was correctly cloned into protein expression vectors.The experiment achieved the pMXB10-TGA2 protein expression vectors containing TGA2 transcription factor.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2011年第5期47-52,共6页
Journal of Hebei Agricultural University
基金
北京市教委面上项目资助(KM200910020014)
北京市园林绿化局治沙办项目资助(2008)
关键词
TGA2转录因子
SAR
广谱抗病
载体构建
TGA2 transcription factor
systematic acquired resistance： disease-resistance to broad-range of pathogens
construction of protein expression vector
