摘要
利用β-葡萄糖苷酶研究了黄芩苷酶解的影响因素及其动力学特征参数,以酶解率为指标考察了黄芩苷酶解的影响因素,并由反应速率计算了酶催化动力学参数rmax和Km值,实验得出较优的酶解条件为:黄芩苷∶酶液为0.1g∶20mL,pH 4.6,55℃搅拌1.5h,酶解率达70%左右,且Km为5.03g.L-1,rmax为0.216g.L-1.min-1.黄芩苷与β-葡萄糖苷酶亲和力强,酶解反应较易进行,在适宜条件下能得到很好的酶解效果.因此,进一步优化酶解条件有望获得更具实用性的酶解工艺.
In order to study factors and enzyme kinetic parameters of the baicalin′s enzymolysis by the β-glucosidase,to study the enzymolysis factors with the enzymolysis rate as an indicator and calculate the kinetic parameters rmax and Km by the enzymatic reaction rate.The better enzymolysis conditions are as follows： baicalin 0.1 g,enzyme solution 20 mL,stir the mixed solution for 1.5 h at the conditions of pH 4.6,55 ℃,the enzymolysis rate is up to 70%,Km is 5.03 g·L-1,rmax is 0.216 g·L-1·min-1,baicalin and β-glucosidase have a strong affinity.The enzymatic reaction is feasible and the effective under optimum conditions.Therefore,further optimization of the enzymolysis conditions is expected to be more pratical enzymatic process.
出处
《陕西科技大学学报（自然科学版）》
2011年第5期24-26,30,共4页
Journal of Shaanxi University of Science & Technology
