摘要
目的研究姜黄素预处理对肢体缺血再灌注肺损伤大鼠肺内炎症反应的影响。方法选取成年♂SD大鼠,建立大鼠肢体缺血2 h再灌注3 h肺损伤模型。随机分为5组(各12只):假手术组及模型组,分别给予等容量生理盐水;3个浓度姜黄素预处理组,分别于缺血前2 h经腹腔注射姜黄素501,00,200 mg.kg-1。测定每组肺组织湿/干重比(W/D),髓过氧化物酶(MPO)活性、肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)含量以及核因子-κB p65(NF-κB p65)的蛋白表达。结果与假手术组比较,模型组的肺组织W/D与MPO活性、TNF-α和IL-6含量并使NF-κB p65表达均明显升高;姜黄素预处理组可剂量依赖地降低肺组织W/D与MPO活性、TNF-α和IL-6含量并使NF-κB p65表达升高。结论姜黄素预处理能减轻肢体缺血再灌注所致的大鼠肺内炎症反应,其机制可能与抑制NF-κB激活、从而减少TNF-α和IL-6介导的中性粒细胞聚集有关。
Objective To investigate the effects of curcumin pretreatment on pulmonary inflammatory response in lung injury induced by ischemia-reperfusion（I/R） of hind limbs in rats.Methods Sixty adult male SD rats were randomly divided into 5 groups（n=12,each）： sham group;model group;three contents curcumin pretreatment groups received curcumin 50,100,200 mg·kg-1 respectively via intraperitoneal injection 2 h before ischemia.I/R of hind limbs was produced by occlusion of bilateral femoral arteries for 2 h followed by 3 h reperfusion in model and curcumin pretreatment groups.The animals were killed at the end of 3 h reperfusion and their lungs were immediately removed for determination of wet/dry weight（W/D） ratio,myeloperoxidase（MPO） activity,tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） content.The lung was also assessed for expression of nuclear factor-κB p65（NF-κB p65） protein using Western blot analysis.Results Lung W/D ratio,MPO activity,TNF-α and IL-6 content were significantly increased in model group as compared with sham group.Lung W/D ratio,MPO activity,TNF-α and IL-6 content were significantly attenuated in the curcumin pretreatment groups as compared with model group in a dose-dependent manner.The expression of NF-κB p65 protein was significantly lower in the curcumin pretreatment groups than in model group in a dose-dependent manner.Conclusion Curcumin pretreatment attenuates pulmonary inflammatory response caused by I/R of hind limbs in rats due to suppressing the activation of NF-κB and subsequent accumulation of neutrophils mediated by TNF-α and IL-6.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2011年第10期770-772,792,共4页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(30600631)
沈阳市科学技术计划基金资助项目(F10-205-1-77)
沈阳医学院优秀人才启动基金资助项目(20103051)
