摘要
为探讨酶解小麦蛋白(Hydrolyzed wheat gluten,HWG)对南美白对虾Litopenaeus vannamei生长性能及免疫机能的影响,试验选用体质量为3.0～3.5 g的南美白对虾750尾,随机分为5个处理组,每组3个重复,每个重复50尾,饲养于水族箱内.处理1:对照组(基础日粮);处理2:基础日粮+0.05%HWG;处理3:基础日粮+0.10%HWG;处理4:基础日粮+0.15%HWG;处理5:基础日粮+0.20%HWG.试验期为53 d.结果表明:1)0.05%HWG组、0.15%HWG组和0.20%HWG组增质量率与对照组相比分别提高了10.23%、7.21%和2.69%,0.15%HWG组饵料系数与对照组相比有下降趋势(P=0.07),0.20%HWG组成活率显著高于对照组(P<0.05);2)HWG组血清中酚氧化酶活性均高于对照组(P>0.05),0.20%HWG组血清中溶菌酶活性显著高于0.05%HWG组(P<0.05),0.10%HWG组和0.15%HWG组肝胰腺超氧化物歧化酶活性与对照组相比分别提高了26.79%和20.19%,0.20%HWG组谷胱甘肽过氧化物歧化酶与对照组相比有提高的趋势(P=0.09),对照组肝胰腺中丙二醛含量高于HWG组(P>0.05).以上结果提示:在南美白对虾日粮中添加HWG能够提高南美白对虾的成活率,具有降低饵料系数和改善免疫机能的趋势.
The study was conducted to determine the effects of dietary Hydrolyzed Wheat Gluten （HWG） supplement on growth performance and immunity of Litopenaeus vannamei. A total 750 Litopenaeus vannameis, with an average initial body mass of 3.0 - 3.5 g, were randomly allocated to 5 treatments, each of which having 3 polyethylene tanks of 50 Litopenaeus vannameis per tank. The experiment lasted for 53 days. The Litopenaeus vannameis per treatment were respectively fed on one of the experimental diets supplemented with 0%, 0. 05%, 0. 10%, 0. 15% and 0. 20% HWG. The results showed that compared with the control group, mass grain rate（MGR） of the 0. 05% HWG, 0. 15% HWG and 0. 20% HWG group were increased by 10. 23%, 7.21% and 2. 69% respectively; feed conversion rate（FCR） of 0. 15%HWG group tended to decrease （P =0. 07） ; survival rate （SR） of 0. 20% HWG group was significantly higher than that of control group （P 〈 0. 05 ）. The activity of phenoloxidase （PO） in serum increased with HWG supplementation, and the activity of lysozyme （LSZ） in serum of 0. 20% HWG group was significantly higher than that of 0. 05 % HWG group （ P 〈 0.05 ）. Compared with the control group, the activities of superoxide dismutase （SOD） in hepatopancreas of the 0. 10% HWG and 0. 15% HWG group were increased by 26. 79% and 20. 19% respectively and glutathione peroxidase （GSH-PX） of 0. 20% HWG group increased as well （ P = 0.09 ）. The malondialdehyde （MDA） content of control group was higher than that of HWG groups in hepatopancreas. These results suggested that HWG could not only increase the SR,but also tend to decrease FCR and improve immunity.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2011年第4期86-90,共5页
Journal of South China Agricultural University
基金
"十一五"国家科技支撑计划(2006BAD12B06)
关键词
酶解小麦蛋白
南美白对虾
生长性能
免疫机能
hydrolyzed wheat gluten
Litopenaeus vannameis
growth performance
immunity
