摘要
目的研究地鳖虫纤溶活性蛋白(fibrinolytic proteinsof Eupolyphaga sinensis,EFP)对肿瘤细胞凋亡的诱导作用及其机制。方法采用纤溶平板法检测了EFP纤溶活性;采用流式细胞术检测了EFP高、中、低浓度对小鼠S180和H22实体瘤组织细胞凋亡的诱导作用;采用免疫组织化学法检测了Caspase-3的表达;采用TRAP-PCR银染法检测了腹水瘤组织端粒酶活性。结果 EFP对S180和H22实体瘤有一定的抑瘤作用,对H22和S180腹水瘤细胞端粒酶活性有一定降低作用,可提高Caspase-3表达水平,诱导细胞凋亡。结论 EFP具有诱导S180和H22肿瘤细胞凋亡作用。
Aim To study the induction effects of fibrinolytic proteins isolated from Eupolyphaga sinensis（EFT） on the apoptosis of tumer S180 and H22 cells in mice and its mechanisms.Methods The molecular weight of EFP was detected by sulfate-polyacrylamide gel electrophoresis analysis.The fibrin plate assay was used to test the fibrinolytic activity of EFP.Tumor bearing mouse models（solid and ascites tumors of S180 and H22） were constructed by injecting tumor cells.Cell apoptotic rate was detected by flow cytometry following annexin-V/PI staining.Caspase-3 levels of solid tumors S180 and H22 were measured by spectrophotometry.Effects of EFP on telomerase activity of H22 and S180 ascites tumor cells was detected by telomeric repeat amplification protocol（TRAP）-silver staining.Results EFP had the effects of inducing apoptosis,raising the level of Caspase-3 of solide tumer S180 and H22,and decreasing the telomerase activity of ascites tumors of S180 and H22.Conclusion EFP can induce cell apoptosis of S180 and H22 cells.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2011年第10期1387-1391,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30772739)
广东工业大学轻工化工学院211工程培育资助项目(No20091015)
