摘要
Inoculated with conventional anaerobic activated sludge, the Anammox process was successfully developed in an anaerobic rotating biological contactor （AnRBC） fed with a low ratio of CfN synthetic wastewater. Operated in a single point feed mode, the AnRBC removed 92.1% （n = 126） of the influent N at the highest surface load of 12 g/（m2.day）. The biomass increased by 25% and 17.1 g/（m2·day） of maximum N removal surface load was achieved by elevating flow rate with another feed point. Fluorescence in situ hybridization and polymerase chain reaction analysis indicated that the Anammox genus Candidatus Kuenenia stuttgartiensis dominated the community. Both Anammox and denitrifying activity were detected in biofilm by the application of microelectrodes. In the outer layer of the biofilm （0-2500 μm）, nitrite and ammonium consumed simultaneously in a ratio of 1.12/1, revealing the occurrence of Anammox. In the inner layer （〉 2500 μm）, a decrease of nitrate was caused by denitrification in the absence of nitrite and ammonium.
Inoculated with conventional anaerobic activated sludge, the Anammox process was successfully developed in an anaerobic rotating biological contactor （AnRBC） fed with a low ratio of CfN synthetic wastewater. Operated in a single point feed mode, the AnRBC removed 92.1% （n = 126） of the influent N at the highest surface load of 12 g/（m2.day）. The biomass increased by 25% and 17.1 g/（m2·day） of maximum N removal surface load was achieved by elevating flow rate with another feed point. Fluorescence in situ hybridization and polymerase chain reaction analysis indicated that the Anammox genus Candidatus Kuenenia stuttgartiensis dominated the community. Both Anammox and denitrifying activity were detected in biofilm by the application of microelectrodes. In the outer layer of the biofilm （0-2500 μm）, nitrite and ammonium consumed simultaneously in a ratio of 1.12/1, revealing the occurrence of Anammox. In the inner layer （〉 2500 μm）, a decrease of nitrate was caused by denitrification in the absence of nitrite and ammonium.
基金
supported by the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0853)
the State Commission of Science Technology of China (No. 2009ZX07212-002)
the National Natural Science Foundation of China (No. 51108367)
the State Key Laboratory of Western Architecture and Technology (No. 10KF08)
the Provincial Key Laboratory Projects of Environmental Engineering of Education Department of Shaanxi Province (No. 2010JS028)
the Foundation of Shaanxi Educational Committee (No. 11JK0759)
