摘要
以平均体质量为(162±26)g雌性三疣梭子蟹为试验对象,注射组从游泳足第一关节基膜注射200μL浓度为2.4×107 CFU/mL的溶藻弧菌菌悬液,对照组注射等量无菌生理盐水。各组在注射0、24、48、72、96 h后随机取9只三疣梭子蟹,从游泳足基膜部位抽取血淋巴,测定血淋巴的血细胞总数、大颗粒细胞比例、血清蛋白、酸性磷酸酶、碱性磷酸酶、一氧化氮和诱导型一氧化氮合成酶等非特异性免疫指标。结果表明,遭受低剂量的溶藻弧菌侵袭后,三疣梭子蟹血细胞总数迅速降低(P<0.05),与对照组相比降低36%,而大颗粒细胞比例则先上升(P<0.05),72 h后恢复至正常水平;血清蛋白含量则无明显变化;血清中酸性磷酸酶、碱性磷酸酶活性也迅速增加,并分别在24、72 h达到峰值(P<0.01,P<0.05),在96 h后两种酶的活性均恢复至对照组水平;血清中一氧化氮含量及诱导型一氧化氮合成酶活性则稳步增加,并在96 h达到峰值(P<0.01)。研究证实低剂量的溶藻弧菌能刺激三疣梭子蟹调动血细胞、酸、碱性磷酸酶、一氧化氮合成酶系统进行免疫防御,但是各非特异性免疫指标大多具有明显的时间效应,响应速度有较大差异。
In this study,the alterations of some typical hematoimmunological parameters in the swimming crab Portunus trituberculatus infected with low dose Vibrio alginolyticus were investigated.Healthy crabs with an average weight of（162±26）g were divided randomly into injected and control groups.Each crab was injected with 200 μL（2.4×107 CFU/mL）V.alginolyticus in injected group,and with 200 μL 0.85% sterile saline solution in control group.Then haemolymph samples were drawn from each crab at hours 0,24,48,72 and 96 post injection for testing hematoimmunological parameters including total hemocyte count（THC）,proportion of large granular cells,total serum protein concentration,acid phosphatase（ACP）,alkaline phosphatase（AKP）,nitric oxide（NO）and inducible nitric oxide synthase（iNOS）.THC of injected crabs was significantly 36% lower than in control group and touched the bottom post injection 24 h（P0.05）.Also,in injected groups,the proportion of large granular cells increased significantly at 24 h and 48 h（P0.05）,and returned to the same level at 72 h and 96 h.Noticeably,there was no significant difference in the injected and control groups in PC,although the numerical value of injected group waved during the test（lower at 24 h,48 h and higher at 72 h,96 h than control group）.The ACP activity,after injection,increased very significantly at 24 h,48 h（P0.01）and 72 h（P0.05）,then decreased to the control level at 96 h.The AKP activity increased gradually after injection,and noticeably,there was no significant difference with control group until at 48 h and 72 h（P0.05）,then decreased to the control level at 96 h.The NO concentration and iNOS activity of injected group shared the same pronounced increasing,there was no significant difference with control group until 72 h（P0.01 for NO and P0.05 for iNOS）and 96 h（P0.01 for NO and iNOS）,especially,the NO concentration in injected group was three times higher than that in control group at 96 h.All the results indicated hema
出处
《水产学报》
CAS
CSCD
北大核心
2011年第9期1392-1398,共7页
Journal of Fisheries of China
基金
浙江省自然科学基金项目(Y3090402)
浙江省科技厅项目(2010R50025
2010F20006)
浙江海洋学院"重中之重"学科建设开放课题(20100211)
关键词
三疣梭子蟹
溶藻弧菌
血淋巴
酸性磷酸酶
碱性磷酸酶
一氧化氮
一氧化氮合成酶
Portunus trituberculatus
Vibrio alginolyticus
haemolymph
acid phosphatase
alkaline phosphatase
nitric oxide
inducible nitric oxide synthase
