摘要
背景:已有实验报道部分骨形态发生蛋白能够诱导间充质干细胞等向成骨细胞的分化。目的:评估骨形态发生蛋白9定向诱导骨骼肌源性干细胞成骨分化的能力。方法:采用Ⅰ型胶原酶和胰蛋白酶二步消化法提取兔骨骼肌源性干细胞,用重组腺病毒的方法将骨形态发生蛋白9导入细胞,通过碱性磷酸酶定量测定、钙盐沉积实验观察骨形态发生蛋白9对于骨骼肌源性干细胞成骨分化的定向诱导作用。结果与结论:成功分离培养兔骨骼肌源性干细胞,免疫细胞化学染色80%以上的细胞呈Sca-1阳性。骨形态发生蛋白9能诱导骨骼肌源性干细胞碱性磷酸酶的表达,且能够促进细胞的钙盐沉积。提示,骨形态发生蛋白9具有定向诱导骨骼肌源性干细胞分化成骨的能力。
BACKGROUND： Previous studies found that some bone morphogenetic proteins can induce mesenchymal stem cells to differentiate into osteoblasts. OBJECTIVE： To assess the capacity of bone morphogenetic protein 9 to induce the differentiation of skeletal muscle-derived stem cells into osteoblasts. METHODS： Rabbit skeletal muscle-derived stem cells were obtained by the two-step method of collagenase-Ⅰ and trypsin, and transfected by bone morphogenetic protein 9 through recombinant adenoviruses. Then, the effect of bone morphogenetic protein 9 on the differentiation of skeletal muscle-derived stem cells into osteoblasts was observed by testing the alkaline phosphatase quantitation and calcium deposition. RESULTS AND CONCLUSION： Muscle-derived stem cells were successfully isolated from the rabbit and over 80% of muscle-derived stem cells showed Sca-1 positive immunoreactivity. Bone morphogenetic protein 9 induced alkaline phosphatase activities in skeletal muscle-derived stem cells and promoted calcium deposition of the cells. From the above, bone morphogenetic protein 9 has the capability to induce the differentiation of skeletal muscle-derived stem cells into osteoblasts.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第32期5971-5974,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
重庆市科委基金(CSTC
2008BB5078)
国家自然科学基金(30772211)
国家自然科学基金(31000434)~~
