摘要
目的探讨临床常见真菌感染的种类及其耐药性特点,为临床感染性疾病提供病原学诊断和合理使用抗真菌药物的依据。方法各种临床标本经常规方法采集,用血平板和沙保弱平皿培养、分离、纯化,血清牙管生长试验,法国生物梅里埃API-20CAUX酵母样真菌鉴定板条鉴定,ATB-Fungus板条进行药敏试验。结果共分离出281株16种真菌,其中主要为白假丝酵母菌164株,占58.4%,热带假丝酵母菌47株,占16.7%,毛霉菌13株,占4.6%。分离的菌株对两性霉素B(AMB)和5-氟胞嘧啶(5-FC)的敏感率很高,而对伊曲康唑(ITR)和氟康唑(FCA)的耐药性率较高。结论临床微生物室须提高真菌的检测手段,重视真菌的培养鉴定和药敏试验,以指导临床合理使用抗真菌药物。
Objective To discuss the normal kinds of yeast-like fungi causing infection and the drug resistance status,and to provide guidance for etiologic diagnosis and suitable anti-fungus management.Methods The fungi were cultured with normal method,then cultured,separated,purified with blood and sabouraud′s culture medium.The bud tube was grown in experimental serum.Then the fungi was isolated and indentified with API-20CAUX indentification system.The antifungal sensitivity was detected with ATB-Fungus trip.Results Totally 16 species were identified from 281 strains of fungus,predominantly Candida abicans（58.4%）,C.tropicialis（16.7%）,Mucor（4.6%）.The isolated fungi were rather sensitive to amphotericin band fluconazole,but drug resistance rate to itraconazole and 5-flucytosine was high.Conclusion Isolation and anti-fungal sensitivity tests should be stressed for clinical microorganism room and clinical selection of reasonable anti-fugals.
出处
《检验医学与临床》
CAS
2011年第20期2488-2489,共2页
Laboratory Medicine and Clinic
关键词
真菌
感染
耐药性
fungi
infection
drug resistance
