摘要
目的:确定制首乌多糖的优化提取工艺。方法:以制首乌多糖提取率为指标,在单因素试验基础上选取试验因素与水平,采用响应面分析法优化提取工艺条件,考察料水比、浸提温度、浸提时间三因素对制首乌多糖提取率的影响。结果:制首乌多糖的最佳提取工艺为:料水比1:19.6(W:V),提取温度89℃,提取时间2.51h。在最佳工艺条件下,制首乌多糖的实际1次提取率可达9.306%。结论:该工艺提取率高、操作简便,易于工业化生产。
Objective： To optimize the extraction process of polysaccharides from Radix Polygoni Multiflori Praeparatacum.Methods： On the basis of single factor experiments,a three-level,three-variable Box-Behnken design was used to optimize the extraction parameters（ratio of water to raw material,extraction time and extraction temperature） of polysaccharides by hot water extraction.Results：The optimum extraction condition was as follows： the ratio of material to water of 1：19.6（W： V）,extraction temperature of 89 ℃ and extraction time of 2.51 h.Under those optimum conditions,the experimental yield was 9.306%.Conclusion：The method was simple,rapid and reliable.
出处
《数理医药学杂志》
2011年第5期568-571,共4页
Journal of Mathematical Medicine
基金
浙江省大学生科技创新创业孵化项目(No.2010R410055)
浙江中医药大学药学院学生课外科研基金项目(No.2009027)
关键词
制首乌
多糖
提取
响应面分析
Radix Polygoni Multiflori Praeparatacum
Plysaccharides
Extraction
Response surface methodology
