摘要
建立辽细辛不同药用部位中马兜铃酸A的含量测定方法并检测其含量。分别采用索氏提取法、超声提取法两种方法对不同产地辽细辛进行前处理,采用反相高效液相色谱法(RP-HPLC)对辽细辛中马兜铃酸A进行测定,色谱柱为XDB-C18(4.6mm×150mm,5μm),流动相为甲醇-水-冰醋酸(70∶29∶1),检测波长为316nm,柱温为室温,流速为1ml/min。分析样品在10分钟内可全部出峰,且在8小时测定时间内稳定性良好;马兜铃酸A含量在20.4ng～102ng范围内相关系数R=0.9999,在2.04ng～20.4ng范围内相关系数R=0.9960,相对标准偏差(RSD)为1.2%。该方法简便快速,线性关系和精密度良好,适用于辽细辛马兜铃酸A含量的测定,所测产地的辽细辛质量较好。
The paper presents a quantitative determination of aristolochic acid A in Asarum Heterotropoides Fr. Schmidt Var. Mand- shuricum （Marim） Kitag. As a treatment, we adopt the ultrasonic extraction and saxhlet extraction respectively used to asarums produced from different origins. And adopt the reversed phase HPLC used to test the aristolochic acid A in asarum. Take XDB - C18 （4. 6mm×150mm, 5μm） as the chromatographic column, and methanol- water- glacial acetic acid （70：29：1 ） as the mobile phase with wave length 316 nm, flow speed lml/min, and room temperature. The correlation coefficient is 0. 9999 for content of aristolochic acid randes from 20.4 ng to 102 ng. The standard relative deviation is 1.2%. This method is simple, rapid, and has linear relation and precision, which is suitable for determination of aristolochic acid in asarum.
出处
《辽宁科技学院学报》
2011年第3期19-21,共3页
Journal of Liaoning Institute of Science and Technology
基金
辽宁省教育厅科技研究资助项目(2009A382)
关键词
辽细辛
高效液相色谱法
马兜铃酸A
测定
Afistolochic Acid Acid in Asarum Heterotropoides Fr. Schmidt Var. Mandshuricum （Mafim） Kitag
HPLC
Aristolochicacid
Determination
