摘要
对分离出的链格孢菌株用ISSR和RAPD分子标记技术分析研究其遗传多样性,比较2种分子生物学方法在链格孢遗传分析中的优劣,为研究烟草赤星病菌遗传多样性及烟草抗病品种的培育奠定基础。采用ISSR和RAPD分子标记方法对来自不同地区的28份烟草赤星病菌进行遗传多样性分析,筛选出10个ISSR引物和10个RAPD引物;ISSR扩增出多态性条带112条,多态性条带比率为86.82%,菌株间相似性系数为0.53～0.97;RAPD引物扩增出多态性条带70条,多态性条带比率为81.39%,菌株间相似性系数为0.57～0.94。用SPSS17.0软件对2种标记遗传距离进行相关性分析,发现2种分子标记结果呈显著正相关,表明2种分子标记方法都适合于烟草赤星病菌遗传多样性研究,ISSR是一种多态性优于RAPD的标记技术。根据2种标记的结果,利用NTSYS软件按UPGMA方法进行聚类分析,发现烟草赤星病菌遗传多样性与地理差异没有显著相关性。
Separate the Alternata strains and study the genetic diversity using ISSR and molecular markers RAPD analysis,compared with two kinds of molecular biology method in Alternata genetic analysis,the good and bad for the study of Alternata genetic diversity and tobacco resistant varieties of cultivating lay the foundation.ISSR and RAPD molecular markers were used to analysis the genetic diversity of 28 A.alternate.Through the primers tested,10 ISSR primers and 10 RAPD primers were selected for their reproducibility and high polymorphism.112 polymorphic bands were amplified by ISSR.The ratio of polymorphic band was 86.82%.The coefficient of similarity between strains was 0.53-0.97.While 70 polymorphic bands were amplified by RAPD and the ratio of polymorphic band was 81.39%.The coefficient of similarity between strains was 0.53-0.94.The correlation of genetic distance of two markers was used analyzed with SPSS 17.0 software.The results proved that these two markers were significantly correlated.The results revealed that lSSR and RAPD markers were suitable for assessment of genetic diversity of A.alternata.The ISSR marker was superior to the RAPD marker.The results reveal that there was little correlation between geographic origin and genetic diversity of A.alternate using NTSYS program by UPGMA cluster analysis.
出处
《中国农学通报》
CSCD
北大核心
2011年第24期262-267,共6页
Chinese Agricultural Science Bulletin
基金
中烟公司科技计划项目"烟草病虫害智能远程诊断及GIS监测预警系统研究及应用"(09YN005)
中烟公司科技计划项目"中国烟草主要病虫害标本资源库的构建"(2010YN18)
中烟公司科技计划项目"云南烟草有害生物调查研究"(2010YN19)
