摘要
目的探讨人类不同成熟阶段的卵母细胞及体外受精发育阻滞胚胎中的组蛋白乙酰化水平的变化规律。方法收集接受卵胞浆内单精子注射技术(IC SI)治疗的患者废弃的卵母细胞生发泡(G V)期、第一次减数分裂中期(MⅠ期)和经体外成熟培养发育至第二次减数分裂中期(MⅡ期)的卵母细胞,采用间接免疫荧光染色方法对人类不同成熟阶段的卵母细胞及体外受精阻滞胚胎中的组蛋白的(H 4K12和H 3K9)乙酰化水平进行检测。结果人类G V期卵母细胞中组蛋白H 4K12和H 3K9均强表达。74.1%的MⅠ期卵母细胞中H 4K12和H 3K9的乙酰化水平显著降低。79.2%的MⅡ期卵母细胞中组蛋白H 4K12乙酰化呈微弱表达或不表达,而来源于高龄妇女的MⅡ期卵母细胞仍维持较高的H 4K12乙酰化水平。在MⅡ期卵母细胞中几乎检测不到H 3K9的乙酰化表达。83.3%的发育阻滞胚胎中组蛋白H 4K12和H 3K9的乙酰化水平较高。结论人类卵母细胞的成熟经历了一个组蛋白乙酰化到去乙酰化的动态修饰过程,体外受精后又重新建立新的组蛋白乙酰化修饰。
【Objective】 To investigate dynamic changes pattern of histone acetylation in human oocytes of different meiotic maturation stages and development arrested embryos in vitro fertilization.【Methods】 Human oocytes of immaturation（GV and MⅠ） and in vitro maturation oocytes were collected from standard ICSI treatments.Oocytes of different maturation stages and development blocked embryos in vitro fertilization were analyzed for acH4K12 and acH3K9 by indirect immunofluorescence staining.【Results】 Human GV stage oocytes showed intense staining of H3 and H4 acetylated lysine residues（H4K12 and H3K9）.74.1% of MⅠ stage oocytes in the level of chromatin acetylated was significantly decreased at H4K12 and H3K9.79.2% of MⅡ stage oocytes showed faint or no staining at H4K12.However,MⅡ oocytes derived from advanced maternal age still kept intense staining at H4K12.All MⅡ oocytes hardly detected expression of H3K9.83.3% of development arrested embryos derived from in vitro fertilization showed intense staining both H4K12 and H3K9.【Conclusions】 Dynamic modification process from histone acetylation to deacetylation appeared during human oocytes in vitro maturation.New histone acetylation pattern was reconstructed after in vitro fertilization.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2011年第25期3125-3128,共4页
China Journal of Modern Medicine
基金
国家自然科学基金资助项目(No:30800650)
湖南省自然科学基金资助项目(No:09JJ4010)
