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PCR-DGGE实验技术在畜牧科学研究中的应用 被引量:1

Application of PCR-DGGE experimental techniques in animal research
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摘要 本文综述了以变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)为主要手段结合聚合酶链式反应(polymerase chain reaction,PCR)实验技术的基本原理、主要实验流程,优缺点以及它在畜禽肠道等微生物的群落结构和多样性研究的最新动态。 In this article ,the basic principle ,main work flow,advantages and limits ,and the latest developments of animal intestinal community structure and diversity about PCR-DGGE were introduced.
出处 《中国饲料》 北大核心 2011年第20期35-38,42,共5页 China Feed
基金 宁波市重大科技攻关计划项目(2008C10023)
关键词 变性梯度凝胶电泳:聚合酶链式反应:微生物 denaturing gradient gel electrophoresis (DGGE) polymcrase chain reaction (PCR) microorganism
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