摘要
以白首乌茎段、叶片、茎尖和带腋芽茎段为外植体,进行离体培养植株再生研究,结果表明:适合初代诱导的培养基为:MS+6-BA1.0 mg.L-1+NAA0.1 mg.L-1,诱导率可达100%;最佳外植体是带腋芽茎段;芽苗增殖的最佳培养基为:MS+KT1.5 mg.L-1+NAA0.05 mg.L-1,增殖系数为5.6;生根培养基为:1/2 MS+NAA0.5 mg.L-1,生根率为93.3%。
Tender stem, leaves, shoot apex , and stem with axillary bud of Cynanchum auriculatum were used as explants in tissue culture and plant regeneration had been studied. The results indicated that the best explants was stem with axillary bud. The most appropriate medium for the original induction was MS ＋ 6-BA 1.0 mg· L^-1 ＋ NAA 0. 1 mg·L^-1 ,with the induction rate of 100%. The optimum medium for muhiplication of buds was MS ＋ KT 1.5 mg · L^-1 ＋ NAA 0. 05 mg -L^-1 ,and the coefficient of multiplication was 5.6. The medium for rooting was 1/2 MS ＋ NAA 0. 5 mg -L^-1 and the rooting rate reached 93.3%.
出处
《浙江农业学报》
CSCD
北大核心
2011年第5期920-923,共4页
Acta Agriculturae Zhejiangensis
关键词
白首乌
组织培养
植株再生
愈伤组织
Cynanchum auriculatum
tissue culture
plant regeneration
callus
