摘要
采用RAPD分子标记技术对恩施州8个不同产地的川续断进行了遗传多样性研究.从50个引物筛选出5个条带清晰、多态性明显并且重复性好的的引物,共扩增出53条DNA片段,利用NTSYS软件进行聚类分析,以相似系数0.85为标准,可将所有品种分为3类,第Ⅰ大类包括建始县龙坪、利川市元堡、恩施市鱼塘坝上坝、恩施市鱼塘坝硒矿区、巴东县绿葱坡;第Ⅱ包括鹤峰县太平乡和鹤峰县五里坪;恩施市双河独立分为一类.本研究可为川续断的鉴别提供一定参考和依据.
Genetic diversity of Dipsacus asper of eight different areas in Enshi by using RAPD molecular markers.5 among 50 primers were selected to amplify about 53 segments DNA.Cluster analysis using NTSYS software,a similar coefficient of 0.85 as the standard,all varieties can be divided into three categories.GroupⅠincluding Longping,Yuanbao,Yutangba embankments,the Se mine enriched area of Yutangba,Lvcongpo;GroupⅡincluding Taiping and Wuliping;Shuanghe of Enshi City is divided into a class of independent.This study can provide a reference and basis for the identification of Dipsacus asper.
出处
《湖北民族学院学报（自然科学版）》
CAS
2011年第3期274-277,共4页
Journal of Hubei Minzu University(Natural Science Edition)
基金
国家民委科研项目(08HB06)
湖北民族学院大学生创新项目(2010)
关键词
道地药材
川续断
RAPD
聚类
genuine regional drug
Dipsacus asper
RAPD
Clustering
